| 用锚定PCR技术筛选谷子微卫星文库的研究 |
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| 引用本文： | 贾小平,王天宇,黎裕,宋燕春,石云素.用锚定PCR技术筛选谷子微卫星文库的研究[J].吉林农业大学学报,2009,31(4). |
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| 作者姓名： | 贾小平 王天宇 黎裕 宋燕春 石云素 |
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| 作者单位： | 1. 河南科技大学农学院,洛阳,471003
2. 中国农业科学院作物科学研究所,北京,100081 |
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| 基金项目： | 国家自然科学基金项目，IAEA资助项目 |
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| 摘 要： | 本试验将锚定PCR技术用于谷子微卫星富集文库的筛选,结果发现该技术能有效减少假阳性的产生,假阳性率仅为7.7%,该技术还能够最大限度淘汰侧翼无法设计引物的无效微卫星克隆,本研究无效克隆比率仅为3.8%.用锚定PCR技术最终筛选开发出13对多态性微卫星标记,这些标记在4个谷子材料和1个野生种中扩增出等位变异数为2～4.锚定FCR技术与常用的同位素杂交筛选技术及普通PCR技术相比,更能降低假阳性克隆和无效微卫星克隆的产生,是一种理想的微卫星文库筛选技术.
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| 关 键 词： | 锚定FCR 微卫星 文库筛选 |
Study on Filtering Microsatellite Library of Foxtail Millet by Anchored PCR Technology |
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| JIA Xiao-ping,WANG Tian-yu,LI Yu,SONG Yan-chun,SHI Yun-su.Study on Filtering Microsatellite Library of Foxtail Millet by Anchored PCR Technology[J].Journal of Jilin Agricultural University,2009,31(4). |
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| Authors: | JIA Xiao-ping WANG Tian-yu LI Yu SONG Yan-chun SHI Yun-su |
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| Institution: | JIA Xiao-ping1,WANG Tian-yu2,LI Yu2,SONG Yan-chun2,SHI Yun-su21.College of Agriculture,Henan University of Science , Technology,Luoyang 471003,China,2.Institute of Crop Science,Chinese Academy of Agricultural Sciences,Beijing 100081 |
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| Abstract: | Though microsatellite markers are at present the main tool for diversity study,genetic map construction,QTL locating and marker assistant selection breeding in plants,the process for microsatellite isolation consumed considerable manpower,material resources and money.So it is very important to increase its exploiting efficiency.In this study,the anchored PCR technology was used to filter microsatellite enriched library of foxtail millet.The results showed that anchored PCR could effectively reduce false pos... |
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| Keywords: | anchored PCR microsatellite library filter |
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