减蛋综合征1976病毒单克隆抗体的制备及其初步鉴定

采用经层析方法提纯的减蛋综合征１９７６（ＥＤＳ‘７６）病毒免疫ＢＬＡＢ／ｃ小鼠。最后一次加强免疫后第３ｄ制备脾脏细胞，然后用５０％的聚乙二醇（ＰＥＧ，分子量４００）融合脾脏细胞和ＳＰ２／０骨髓瘤细胞。通过选择培养、特异性抗体检测、筛选和克隆詈笾票赋觯抵攴置诳固宓南赴辏停玻停常停保埃停保春停停保怠Ｎ⒘垦种剖匝椋ǎ龋桑┲っ鳎停保档タ寺】固寰哂醒种苹钚裕龋杉畚叮ǎ欤铮纾玻

PREPARATION OF MONOCLONAL ANTIBODIES AGA-INST EGG DROP SYNDROME 1976 VIRUS AND THEIR PRELIMINARY CHARACTERIZATION

BLAB/C mice were immunized with chromatographically purified EDS'76 virusV205 strain,and splenocytes were prepared three days after the final boosterimmunization. Fusion of lymphocyte and myeloma SP2/0 cell line was carriedout with the fusogen 50% polyethylene glycol(M.W. 4 000).Five antibodysecreting cel lines,named M2,M3,M10,M14 and M15,were finally obtained afterselective cultivation,specific antibody detection and cloning.It was shown thatM15 monoclonal antibody had haemagglutination inhibition activity with a titerof 6(log2)in microplate HI test.All five McAbs were determined to be stronglyspecific to EDS'76 virus with no reaction to IBV,ILTV,influenza A virus,CELOvirus,NDV and IBDV.