| 墨兰离体快繁研究 |
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| 引用本文： | 项艳,於凤安,彭镇华.墨兰离体快繁研究[J].林业科学研究,2003,16(4):434-438. |
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| 作者姓名： | 项艳 於凤安 彭镇华 |
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| 作者单位： | 1. 安徽农业大学森林利用学院,安徽合肥,230036
2. 中国林业科学研究院,北京,100091 |
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| 基金项目： | 中国森林生态网络体系"线"的研究与示范(2002BA516A15) |
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| 摘 要： | 采用墨兰的茎尖和芽为外植体,研究墨兰的组织培养要点。通过对墨兰组织培养中外植体的选择、芽的诱导、继代增殖、壮苗培养、生根诱导和移植进行研究,运用正交试验筛选出芽快速生芽的最佳培养基为:MS+6 BA4.0mg·L-1+NAA1.0mg·L-1+琼脂8g·L-1+蔗糖30g·L-1,生根的最佳培养基为:1/2MS+NAA3.0mg·L-1。分析比较出芽长为1.0cm,叶长为1.5cm,叶数为2～3条的兰花苗有利于生根。
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| 关 键 词： | 墨兰 离体快繁 组织培养 外植体 培养基 试管苗 |
| 文章编号： | 1001-1498(2003)04-0434-05 |
| 收稿时间： | 2002/10/12 0:00:00 |
Tissue Culture of Cymbidium sinensis |
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| XIANG Yan,YU Feng-an and PENG Zhen-hua.Tissue Culture of Cymbidium sinensis[J].Forest Research,2003,16(4):434-438. |
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| Authors: | XIANG Yan YU Feng-an and PENG Zhen-hua |
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| Institution: | Anhui Agricultural University, Hefei230036, Anhui, China;Anhui Agricultural University, Hefei230036, Anhui, China;Chinese Academy of Forestry, Beijing100091, China |
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| Abstract: | The explants of Cymbidium sinensis stems and spores were used to study Cymbidium sinensis tissue culture essentials. It dealed with the selection of explant, induction of proliferation, subculture, rooting and outplanting of Cymbidium sinensis. The results showed that the best medium to induce proliferation was MS+6-BA 4.0 mg·L-1+NAA 1.0 mg·L-1+agar 8 g·L-1+sugar 30 g·L-1, by wielding orthogonal test. While rooting medium was half MS and 3.0 mg·L-1of NAA. At the same time,we obtained by analyzing and contrasting the standard spore to bring about rooting, which offered basis of Cymbidium sinensis, such as enlarging produce, increasing living rate and improving economy benefits correspondingly. |
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| Keywords: | Cymbidium sinensis spore induction proliferation rooting outplanting |
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