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| 秋水仙素诱导离体培养越橘多倍体研究 | |
| 引用本文： | 李晓艳,张志东,李亚东,吴林,刘海广. 秋水仙素诱导离体培养越橘多倍体研究[J]. 东北农业大学学报, 2010, 41(1) |
| 作者姓名： | 李晓艳 张志东 李亚东 吴林 刘海广 |
| 作者单位： | 吉林农业大学园艺学院,长春,130118 |
| 基金项目： | 公益性行业科研专项(nyhyzx07-028,2006-G25);;吉林省科技厅重点项目(2007-5013);;吉林省财政厅农业育种项目 |
| 摘 要： | 试验研究了秋水仙素不同处理方法、浓度和时间对美登、达柔和埃利奥特3个越橘品种试管苗的诱变效果,并采用根尖压片方法和流式细胞仪对诱变材料倍性进行检测。结果表明,采用药剂培养基法处理效果不如茎段浸渍法。经秋水仙素处理的所有品种都发生不同程度的变异,其中达柔用0.1%秋水仙素浸渍24h的处理效果最好,变异率达到22.6%,诱变株系为嵌合体。流式细胞仪测定DNA含量方法与根尖压片方法检测结果一致。 |
| 关 键 词： | 越橘 秋水仙素 多倍体 离体培养 |
Study on polyploid induction of blueberry in vitro with colchicine treatment |
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| Li Xiaoyan,ZHANG Zhidong,LI Yadong,WU Lin,LIU Haiguang. Study on polyploid induction of blueberry in vitro with colchicine treatment[J]. Journal of Northeast Agricultural University, 2010, 41(1) | |
| Authors: | Li Xiaoyan ZHANG Zhidong LI Yadong WU Lin LIU Haiguang |
| Affiliation: | College of Horticulture;Jilin Agricultural University;Changchun 130118;China |
| Abstract: | Take blueberry cultivars Blomidon,Darrow,Elliott as experimental materials,treating them with different methods,time and concentrations of colchicine.The results showed that induction effect through medium with colchicine treatment was inferior to that through immersing axillary buds.With colchicine 0.1% immersing axillary Darrow buds for 24 h,the variation rate was 22.6%.The variation plants were chimeras.The result of counting chromosome of root tip was identical with the analysis of DNA content in plant ... |
| Keywords: | blueberry colchicine polyploid in vitro culture |
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