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| KT对白花草木樨不同外植体愈伤组织培养的影响 | |
| 引用本文： | 毛雅妮,张德罡,孙娟,盛亦兵,杨国锋.KT对白花草木樨不同外植体愈伤组织培养的影响[J].草原与草坪,2009(5):33-34. |
| 作者姓名： | 毛雅妮 张德罡 孙娟 盛亦兵 杨国锋 |
| 作者单位： | 1. 甘肃农业大学,草业学院,甘肃,兰州,730070;青岛农业大学,山东,青岛,266109 2. 甘肃农业大学,草业学院,甘肃,兰州,730070 3. 青岛农业大学,山东,青岛,266109 4. 山东省农业可持续发展研究所,山东济南,250110 |
| 基金项目： | 国家十一五科技支撑项目"牧草倍性育种技术研究"和山东省农业良种工程牧草项目"优质高产牧草和饲料作物新品种选育" |
| 摘 要： | 以改良HS为基本培养基，研究了白花草木樨不同外植体和KT（激动素）浓度对愈伤组织诱导的影响。结果表明：以无菌苗子叶、下胚轴和幼根为外植体，下胚轴出愈率为100．0％，为诱导愈伤组织的最佳外植体；子叶和下胚轴出愈率随KT浓度增大呈抑制作用，在1．0mg／L2，4-D＋0．1mg／LKT时出愈率分别为98．00％和100．00％，为最佳激素浓度配比；幼根出愈率随KT浓度增大先促进后抑制，1．0mg／L2，4-D＋0．3mg／LKT时出愈率为99．33％，为最佳激素浓度配比。 |
| 关 键 词： | 白花草木樨 外植体 KT 愈伤组织 |
Effects of KT on culture tissue of different explants of Melilotus suaveolens |
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| MAO Ya-ni,ZHANG De-gang,SUN Juan,SHENG Yi-bing,YANG Guo-feng.Effects of KT on culture tissue of different explants of Melilotus suaveolens[J].Grassland and Turf,2009(5):33-34. | |
| Authors: | MAO Ya-ni ZHANG De-gang SUN Juan SHENG Yi-bing YANG Guo-feng |
| Institution: | MAO Ya-ni1,ZHANG De-gang1,SUN Juan2,SHENG Yi-bing3,YANG Guo-feng2(1.College of Pratacultural Science,Gansu Agricultural University,Lanzhou 730070,China,2.Qingdao Agricultural University,Qingdao 266109,3.Sh,ong Institute of Agricultural Sustainable Development,Jinan 250100,China) |
| Abstract: | The modified HS was used as a basal medium to study the effects of different explants of Melilotus suaveolens,including aseptic seedling of cotyledon,hypocotyl and radicle,and KT(kinetin) concentrations on callus induction.The results showed that the hypocotyl was the best explant for inducing callus and the callus rate of was 100.0%.The callus induction rates of cotyledon and hypocotyl were inhibited along with the increase ofKT concentration,and under the condition of 1.0mg/L 2,4-D+0.1mg/L KT,their callus... |
| Keywords: | KT |
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