| 中国环颈雉心脏型脂肪酸结合蛋白基因的cDNA克隆及序列分析 |
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| 引用本文： | 吴琼,杨福合,邢秀梅,荣敏. 中国环颈雉心脏型脂肪酸结合蛋白基因的cDNA克隆及序列分析[J]. 特产研究, 2012, 34(1): 1-5,30 |
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| 作者姓名： | 吴琼 杨福合 邢秀梅 荣敏 |
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| 作者单位： | 中国农业科学院特产研究所吉林省省部共建特种经济动物分子生物学国家重点实验室,吉林吉林,132109 |
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| 基金项目： | 国家科技基础条件平台建设项目(2005DKA21102) |
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| 摘 要： | 运用RT-PCR技术,克隆了中国环颈雉心脏型脂肪酸结合蛋白基因的序列,并对其进行了测序及生物信息学分析。结果表明:中国环颈雉H-FABP基因cDNA序列长为402bp,开放阅读框为399bp,编码132个氨基酸,推测的蛋白相对分子质量为14.64KD,等电点为6.30。中国环颈雉H-FABP基因与已报道的鸡、鸭、珍珠鸡等禽类动物的核苷酸和编码氨基酸序列具有很高的相似性。
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| 关 键 词： | 中国环颈雉 心脏型脂肪酸结合蛋白 克隆 序列分析 |
cDNA Cloning and Sequence Analysis of Heart-type Fatty acid binding protein Gene in Chinese Ring-necked Pheasant |
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| WU Qiong , YANG Fu-he , XING Xiu-mei , RONG Min. cDNA Cloning and Sequence Analysis of Heart-type Fatty acid binding protein Gene in Chinese Ring-necked Pheasant[J]. Special Wild Economic Animal and Plant Research, 2012, 34(1): 1-5,30 |
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| Authors: | WU Qiong YANG Fu-he XING Xiu-mei RONG Min |
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| Affiliation: | (Institute of Special Economic Animals and Plants,Chinese Academy of Agricultural Sciences,State Key Laboratory of Special Economic Animal Molecular Biology,Jilin 132109,China) |
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| Abstract: | Heart-type fatty acid binding protein(H-FABP) cDNA was cloned from chinese ring-necked pheasant using RT-PCR method.The cDNA was sequenced and analyzed.The result showed that the cloned cDNA fragment was 402 bp,contained an open-reading frame of 399bp encoding 132 amino acids.The supposed molecular weight of the putative H-FABP protein was 14.64KD,and theoretical PI was 6.30.It indicates that nucleotide sequence and deduced amino acid sequence showed a high homology between chinese ring-necked pheasant and poultry. |
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| Keywords: | Pheasant H-FABP Cloning Sequences analysis |
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