氩离子注入介导甘草总DNA在大叶紫花苜蓿中转化的初步研究

氩离子注入介导乌拉尔甘草总DNA在和田大叶紫花苜蓿中转化,对苜蓿正常生长和发育产生了显著的生物学效应.转基因苜蓿T1代的结实率仅有11.66%,其中发育正常的种子比例为64.29%.对种子发育良好的苜蓿T1代9个单株的根茎分别进行了天然产物的定性检识,结果表明,2个单株的根中含有皂苷类物质.用X射线衍射仪对2个处理的转基因苜蓿进行扫描,结果表明,一种图谱经寻峰处理后,d值相对偏差最大为0.67,大于本实验误差允许范围0.02,说明样品微观结构或成份发生了变化.提取并测定了转基因苜蓿与对照组茎和根中的多糖含量,结果表明,转基因苜蓿茎和根中多糖含量分别为0.890%和4.815%,对照组苜蓿多糖含量分别为1.064%和4.882%,说明转入甘草总DNA的苜蓿多糖的含量均有所降低.

A Prelimary Study on the Transformation of Glycyrrhiza uralensis Genome DNA in Hetian Alfafa Implanted by Argon Ion

The total DNA of Glycyrrhiza uralensis were randomly implanted into Medicago sativa ( Hetian alfalfa ) by argon ion, and induced obvious biological effects on the normal growing and cultivating of the alfalfa. The seed-setting rate of T1 generation transgene alfalfa was only 11.66%, and normal seeds was 64.29%. The qualitative test of the natural products was carried out to the nine individual plant's roots and stems of T 1 generation transgene alfalfa with well developed seeds. The results of natural products detection showed that some saponins were contained in roots of the two individual plants. X-ray diffraction was used to scan medicinal transgenic alfalfa and the diffract gram map were obtained. The polysaccharide of control group and T 1 generation were extracted from roots and stems and determined. The results showed that after searching peaks-processing, the relative error was 0.67, the difference of crystal spacing was greater than the allowed error 0.02, which reflected that the micro-structure or component of the sample changed. The contents of polysaccharide in the transgenic alfalfa's stems and roots were 0. 890%, 4. 815% respectively, compared with that of the common alfalfa's 1.064%, 4.882%. These results reflected that the content of polysaccharide in transgene alfalfa decreased which implanted Glycyrrhiza uralensis total DNA.