山楂（Crataegus pinnatifida Bge.)遗传多样性的RAPD和ISSR标记分析

 利用RAPD和ISSR标记对35份山楂（Crataegus pinnatifida Bge.）资源进行了DNA多态性分析。12个RAPD引物共扩增出110条清晰的谱带，其中89条显示多态性，平均每个引物扩增出7.4条多态性谱带。13个ISSR引物共扩增出110条清晰的谱带，其中94条显示多态性，平均每个引物扩增出7.2条多态性谱带。基于RAPD和ISSR标记，利用UPGMA分别构建了35份山楂资源的聚类树状图。距离系数分别为0～0.62（RAPD）和0～0.64（ISSR），表明山楂具有较高的遗传多样性。

Genetic Diversity of Crataegus pinnatifida Bge. as Evaluated by RAPD and ISSR Markers

The DNA polymorphism of thirty-five hawthorn accessions (Crataegus pinnatifida Bge.) was analyzed using randomly amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers in the present study. Twelve RAPD primers generated a total of 110 clear and reproducible bands, of which 89 were polymorphic, with an average of 7.4 polymorphic fragments per primer. Thirteen ISSR primers generated a total of 110 clear and reproducible bands, of which 94 were polymorphic, with an average of 7.2 polymorphic fragments per primer. The dendrogram based on RAPD and ISSR data were constructed separately by using UPGMA analysis. The distance coefficient of the analyzed materials ranged from 0 to 0.62 and 0 to 0.64 for RAPD and ISSR markers, respectively. Our results indicated that the C. pinnatifida species has high level of genetic diversity.