家蚕Notch信号通路基因的表达研究

目的]研究家蚕Notch信号通路基因的表达情况。方法]在实验室前期工作的基础上,用特异引物对家蚕的Notch信号通路上下游基因进行克隆,并对这些基因在家蚕5龄幼虫不同组织部位的表达进行研究。结果]这些基因在各组织的表达量不同,其中fringe和groucho在家蚕头部表达量较多,在丝腺、精巢、卵巢中的表达量较少;notch在家蚕尾部表达量较少,在其他组织中表达量无明显差别。结论]该研究为进一步研究家蚕Notch信号通路奠定了基础。

Study on Expression of Notch Signaling Pathway Genes in Silkworm

Objective] The aim was to study the expression of Notch signaling pathway genes in silkworm. Method] Based on the previous studies, genes of Notch signaling pathway in silkworm were cloned by the specific primers, and the expression profiles of them in different tis-sues of the fifth instar larvae were analyzed. Result] The expression patterns of Notch signaling pathway genes were different, in which fringe and groucho accumulated more in head of the silkworm and with little expression in silk gland, testis and ovary. While notch had relatively less expression in tail but with constructive expression in other tissues. Conclusion] The study laid a basis on the further research of Notch signa-ling pathway.