| 长穗桑四倍体诱导初步研究 |
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| 引用本文： | 武宇坤,何建,王西木,郭启高,梁国鲁.长穗桑四倍体诱导初步研究[J].热带农业科技,2008,31(2). |
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| 作者姓名： | 武宇坤 何建 王西木 郭启高 梁国鲁 |
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| 作者单位： | 西南大学,园艺园林学院,重庆,400716 |
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| 摘 要： | 以长穗桑为试验材料,对其进行继代增殖及对最佳增殖培养基进行了筛选,并用组织培养技术结合秋水仙素溶液浸泡法以获得四倍体新材料。结果表明:增殖培养基采用MS+BA2.0mg/L+NAA0.2mg/L+蔗糖30mg/L最佳,其最高的增殖系数为3.2。四倍体诱导率达到26.7%,其染色体数目为2n=4x=56,最佳诱导条件为用0.2%的秋水仙素溶液浸泡3d。所获得的四倍体最适生根培养基为1/2MS+BA0.1mg/L+NAA0.05mg/L+30mg/L蔗糖,生根率达到100%。
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| 关 键 词： | 长穗桑 多倍体 组织培养 诱导 |
Preliminary Study on Tetraploid Induction of Morus wittiorum |
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| WU Yu-kun,HE Jian,WANG Xi-mu,GUO Qi-gao,LIANG Guo-lu.Preliminary Study on Tetraploid Induction of Morus wittiorum[J].Tropical Agricultural Science & Technology,2008,31(2). |
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| Authors: | WU Yu-kun HE Jian WANG Xi-mu GUO Qi-gao LIANG Guo-lu |
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| Abstract: | An experiment of Morus wittiorum was carried out in vitro with colchicine solutions to select the favourable proliferation medium for tetraploid. The results indicated that the optimum proliferation medium was MS+BA2.0mg/L+NAA0.2mg/L+sugar 30mg/L. The proliferation coefficient could reach to 3.2 and induction rate of tetraploid was 26.7% with chromosome number of 2n=4x=56. The optimal induction result was obtained by treatment with 0.2% colchicine solution for 3 days.1/2MS+BA0.1mg/L+NAA0.05mg/L+sugar 30mg/L was optimal for rooting, and rooting rate reached 100%. |
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| Keywords: | Morus wittiorum polyploid tissue culture induction |
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