| 茶树发根中茶氨酸和儿茶素类含量的分析 |
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| 引用本文： | 张广辉,梁月荣,陆建良,董俊杰.茶树发根中茶氨酸和儿茶素类含量的分析[J].茶叶科学,2006,26(4):305-309. |
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| 作者姓名： | 张广辉 梁月荣 陆建良 董俊杰 |
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| 作者单位： | 1. 浙江大学茶学系,浙江,杭州,310029;江苏省徐州生物工程学校,江苏,徐州,221006
2. 浙江大学茶学系,浙江,杭州,310029 |
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| 基金项目： | 国家自然科学基金;浙江省科技计划 |
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| 摘 要： | 茶树发根在LG0培养基和添加1
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| 关 键 词： | 茶树 发根培养 茶氨酸 儿茶素 HPLC |
| 文章编号： | 1000-369X（2006）04-305-05 |
| 收稿时间： | 2006-06-20 |
| 修稿时间： | 2006-09-08 |
Analysis of Theanine and Catechins in Hairy Roots of Camellia sinensis |
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| ZHANG Guang-hui,LIANG Yue-rong,LU Jian-liang,DONG Jun-jie.Analysis of Theanine and Catechins in Hairy Roots of Camellia sinensis[J].Journal of Tea Science,2006,26(4):305-309. |
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| Authors: | ZHANG Guang-hui LIANG Yue-rong LU Jian-liang DONG Jun-jie |
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| Institution: | 1. Department of Tea Science, Zhejiang University, Hangzhou 310029, China; 2. Xuzhou Higher Vocational School of Bioengineering, Xuzhou 221006, China |
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| Abstract: | Hairy roots induced by Agrobacterium rhizogenes wild strain 15834 were propagated on LG0 medium and half MS medium containing 1 mg/L IBA to promote rapid growth. Although rapid growth was obtained in half MS media containing 1 mg/L IBA, high content of theanine (23.12 mg/g fresh weight) was only detected in one hairy root clone cultured on LG0 medium and higher than the non-transformed root (18.77 mg/g fresh weight). High content of aspartic acid, glutamic acid, glutamine, histidine and arginine were also detected in this hairy root clones. Medium constitute also affected the contents of amino acid in hairy root clone, the content of theanine were reduced in two hairy root clones when they were cultivated on half MS with 1 mg/L IBA. Low content of simple catechins, catechin (C) and epicatechin (EC), were also detected in hairy roots. |
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| Keywords: | HPLC |
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