| 兴义矮脚鸡GH基因的多态性研究 |
| |
| 引用本文： | 朱丽莉,王闯,傅筑荫,陈彬,张福平.兴义矮脚鸡GH基因的多态性研究[J].中国畜牧兽医,2010,37(12):96-98. |
| |
| 作者姓名： | 朱丽莉 王闯 傅筑荫 陈彬 张福平 |
| |
| 作者单位： | （贵州大学动物科学学院，贵州省动物遗传育种与繁殖重点实验室，贵阳 550025） |
| |
| 摘 要： | 兴义矮脚鸡为贵州特有的地方品种鸡。本研究对兴义矮脚鸡GH基因上第一外显子和部分第一内含子序列直接测序进行多态性分析。结果表明，在扩增出来的777 bp序列中，A、T、G、C碱基的平均含量分别为28.1%、23.9%、24.9%和23.1%。A+T的含量(52%)略高于G+C的含量(48%)；总共发现10个变异位点，占分析位点总数的1.287%，这些变异位点分别为C124T、T319G、T321C、G333A、A364G、A464C、A507C、C508A、T541C和T630C，其中C124T突变点位于5′端调控区，其余突变点都集中在第一内含子上，均为转换，表现较高的转换偏向。
|
| 关 键 词： | 兴义矮脚鸡 GH基因 多态性 |
Polymorphisms of Growth Hormone Gene in Xingyi Bantom |
| |
| ZHU Li-li,WANG Chuang,FU Zhu-yin,CHEN Bin,ZHANG Fu-ping.Polymorphisms of Growth Hormone Gene in Xingyi Bantom[J].China Animal Husbandry & Veterinary Medicine,2010,37(12):96-98. |
| |
| Authors: | ZHU Li-li WANG Chuang FU Zhu-yin CHEN Bin ZHANG Fu-ping |
| |
| Institution: | (College of Animal Science，Guizhou University，Guizhou Key Laboratory of Animal Genetics Breeding and Reproduction，Guiyang，550025，China) |
| |
| Abstract: | Xingyi bantom is a special Guizhou chicken breed native. In the present study, the polymorphism of exon 1 and inton1 of growth hormone(GH) gene about 777 bp was measured with the method of direct sequenceing in Xingyi bantom. The results showed that the complete sequences was A28.1%,T23.9%,G24.9% and C23.1% respectively.A+T（52%）was higher than G+C（48%）.A total of 10 were detected that covering 1.287% of the entire length of the sequence. The mutation sites were C124T, T319G, T321C, G333A, A364G, A464C, A507C, C508A, T541C and T630C. Meanwhile the C124T in 5′-UTR and the others in intron 1.All of them are transformation phenotyping the higher the deviation. |
| |
| Keywords: | Xingyi bantom GH gene polymorphism |
|
| 点击此处可从《中国畜牧兽医》浏览原始摘要信息 |
| 点击此处可从《中国畜牧兽医》下载免费的PDF全文 |
|
