利用沼气废液培养螺旋藻

目的]利用沼液培养螺旋藻。方法]通过向沼液中添加一定浓度的Zarrouk培养液获得了螺旋藻在沼液中的培养技术,采用正交实验优化了培养条件。并通过测定螺旋藻培养过程中,沼液中的氮、磷含量的变化,研究了螺旋藻对沼液的净化效果。结果]螺旋藻不能在直接在沼液中生长,但在其中添加了10%比例的Zarrouk培养液后,螺旋藻生长良好。正交实验显示螺旋藻在沼液中的最佳生长条件为：pH为8、初始密度OD560为0.3,光照强度5 000 lx。螺旋藻对废水中的氮、磷均有较好的清除效果,螺旋藻对活性磷的清除率达到97.1%,对硝态氮的清除率为61.2%,对亚硝态氮的清除率为53.6%。结论]利用沼液培养螺旋藻不仅可以净化环境,而且降低生产螺旋藻成本,这为沼液的资源化处理提供了一条新的高效而经济的途径。

Cultivation of Spirulina in Biogas Wastewater

Objective] The aim was to obtain the way to cultivate Spirulina in biogas wastewater.Method] The cultivating technology of Spirulina in the biogas wastewater was obtained by adding certain proportion of Zarrouk culture to the wastewater,and the cultivating condition was optimized by the orthogonal experiment.The purification of the biogas wastewater by Spirulina was also studied by testing the changes of the concentrations of nitrogen and phosphorus in the wastewater during the cultivation of Spirulina.Result] Spirulina could not grow directly in the biogas wastewater,but also could grow very well after adding 10% of Zarrouk culture to the wastewater.The orthogonal experiment showed that the best growing condition for Spirulina in the wastewater was pH 8,initial optical density 0.3 at 560 nm,illumination 5000 lx.The study also showed that Spirulina had good removal of the nitrogen and phosphorus in the wastewater.The elimination rates of active phosphorus,nitrate nitrogen and nitrite nitrogen by Spirulina reached to 97.1%,61.2%,and 53.6% respectively.Conclusion] It can be feasible to cultivate Spirulina in the biogas wastewater.The cultivation can not only purify environment,but also greatly cut the production cost of Spirulina,which would be an effective and economical way to the resource utilization of the biogas wastewater.