转BADH基因和mtlD基因花生幼苗抗盐性研究

[目的]研究转mtlD基因和BADH基因花生幼苗的抗盐性。[方法]通过根癌农杆菌介导将外源mtlD和BADH基因同时导入花生,并检测植株的鲜重、高度、电导率等指标以判断转基因花生的抗盐性。[结果]PCR和Northern杂交都显示,mtlD和BADH基因已成功整合到花生染色体上;转基因植株的鲜重、高度、细胞膜的完整程度都明显高于对照植株。[结论]mtlD基因和BADH基因在花生中的表达增强了转基因花生的抗盐性。

Study on Salt Resistance of Peanut Seedlings with Transgenic BADH and mtlD

[Objective] The aim was to study the salt resistance of peanut seedlings with transgenic BADH and mtlD.[Method] mtlD and BADH gene are transformed into peanut with the help of Agrobactrium tumefaciens,the salt resistance of transgenic peanut is determined by detecting the plants fresh weight,height and conductivity.[Result] The hybridization of PCR and Northern indicate that the mtlD and BADH gene have been integrated into genome of peanut and expressed in transgenic peanut plants;the values of fresh weight,height and the integrity of the cell membrane increase significantly compared with the control plants.[Conclusion] The expression of the mtlD and BADH gene are able to enhance the salt resistance of peanut.