| 台尔曼忍冬的组织培养与快速繁殖 |
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| 引用本文： | 徐美隆,吴建华,闵丽霞.台尔曼忍冬的组织培养与快速繁殖[J].安徽农业科学,2011,39(23):13977-13978. |
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| 作者姓名： | 徐美隆 吴建华 闵丽霞 |
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| 作者单位： | 国家经济林木种苗快繁工程技术研究中心,银川宁夏,750004 |
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| 基金项目： | 北京市都市产业科技促进课题(Z101105002510005) |
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| 摘 要： | 目的]建立台尔曼忍冬的组织培养和快速繁殖技术体系,为台尔曼忍冬优良株系的快速繁殖提供依据。方法]以台尔曼忍冬未木质化或半木质化茎段为外植体,用不同浓度的NAA与IBA培养基进行组培快繁研究。结果]在MS＋1.0 mg/L 6-BA＋0.1 mg/LNAA培养基上培养8～10 d后开始有芽萌动;MS＋0.8 mg/L 6-BA＋0.1 mg/L NAA培养基对增殖效果较好;生根培养基为1/2MS＋0.8mg/L IBA＋0.1 mg/L NAA,生根率达78.5%。结论]该研究建立的台尔曼忍冬组培快繁体系稳定性好,增殖速度快。
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| 关 键 词： | 台尔曼忍冬 组织培养 快速繁殖 |
Tissue Culture and Rapid Propagation of Lonicerra tellmanniana Spaeth |
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| Institution: | XU Mei-long et al(The National Center of Research and Engineering Technology of Economic Forest Tree Speedy Propagation,Yinchuan,Ningxia 750004) |
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| Abstract: | Objective]To establish the tissue culture and rapid propagation system of Lonicerra tellmanniana Spaeth,so as to provide basis for its rapid propagation.Method]With the non-lignified and semi-lignified stems as explants and different concentrations of NAA and IBA as the medium,the tissue culture and rapid propagation of Lonicerra tellmanniana Spaeth were studied.Result]The young buds could be induced after 8-10 d culture on the MS+1.0 mg/L 6-BA and 0.1 mg/L NAA.The MS medium with 0.8 mg/L 6-BA and 0.1 mg/ L NAA was the best for multiplication culture;the medium for rooting was 1/2MS+0.8 mg/L IBA+0.1 mg/L NAA,the rooting ratio was up to 78.5%.Condusion]The established tissue culture and rapid propagation system for Lonicerra tellmanniana Spaeth had good stability and fast propagation rate. |
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| Keywords: | Lonicerra tellmanniana Spaeth Tissue culture Rapid propagation |
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