| 马铃薯纺锤块茎类病毒检测技术比较与选择 |
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| 引用本文： | 邱彩玲,吕文河,魏琪,吕典秋,李学湛,白艳菊.马铃薯纺锤块茎类病毒检测技术比较与选择[J].中国马铃薯,2013(6):362-364. |
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| 作者姓名： | 邱彩玲 吕文河 魏琪 吕典秋 李学湛 白艳菊 |
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| 作者单位： | [1]黑龙江省农业科学院植物脱毒苗木研究所,黑龙江哈尔滨150086 [2]东北农业大学,黑龙江哈尔滨150030 |
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| 基金项目： | 黑龙江省农业科学院创新工程项目“黑龙江省马铃薯类病毒（PSTVd）致病力分析及品种抗性鉴定”;现代农业产业技术体系专项基金资助项目（CARS-10-P14）;国家科技支撑项目（2012BAD06802）. |
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| 摘 要： | 马铃薯纺锤块茎类病毒(PSTVd)是威胁我国马铃薯生产的重要病害之一,目前有多种技术可应用于PSTVd的检测,如生物学方法、电子显微镜技术、往返聚丙烯酰胺凝胶电泳(R-PAGE)、核酸斑点杂交(NASH)、RT-PCR和FQ RT-PCR。这些方法各有利弊,互相补充,构成了PSTVd的检测技术体系。日常工作中,应根据自身条件及目的等科学地选择适宜的检测技术,确保脱毒种薯/种苗的质量。
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| 关 键 词： | 马铃薯纺锤块茎类病毒(PSTVd) 检测技术 比较与选择 |
Comparison and Selection of Potato Spindle Tuber Viroid Detection Techniques |
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| QIU Cailing,LU Wenhe,WEI Qi,LU Dianqiu,LI Xuezhan,BAI Yanju.Comparison and Selection of Potato Spindle Tuber Viroid Detection Techniques[J].Chinese Potato,2013(6):362-364. |
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| Authors: | QIU Cailing LU Wenhe WEI Qi LU Dianqiu LI Xuezhan BAI Yanju |
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| Institution: | 1. Virus-free Seedling Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin, Heilongjlang 150086, China 2. Northeast Agricultural University, Harbin, Heilongjiang 150030, China ) |
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| Abstract: | Potato spindle tuber viroid （PSTVd） is one of the important diseases that threatens the potato production in China. Now, there are some techniques for detection of PSTVd, such as biological method, electron microscope technique, R-PAGE, NASH, RT-PCR and FQ RT-PCR. These methods have advantages and disadvantages and they can complement each other, comprising a system for PSTVd detection. Daily work should be based on their own conditions and purposes, and the detection method be appropriately chosen to control the virus-free seed/seedling quality. |
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| Keywords: | potato spindle tuber viroid （PSTVd） detection technique comparison and selection |
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