植物离体培养物中鱼藤酮的分离与HPLC测定

毛鱼藤（Ｄｅｒｒｉｓ ｅｌｌｉｐｔｉｃａ （Ｒｏｘｂ．）Ｂｅｎｔｈ．）和西非灰毛豆（Ｔｅｐｈｒｏｓｉａ ｖｏｇｅｌｉｉ Ｈｏｏｋ．ｆ．）离体培养物经９：１ＣＨＣｌ３／ＭｅＯＨ溶剂提取，Ｃ－１８柱层析分离后，在室温条件下以６６：３４ＭｅＯＨ／Ｈ２Ｏ或４２：５８ＣＨ３ＣＮ／Ｈ２Ｏ为流动相，支样品进行反相柱ＨＰＬＣ分析。结果表明，毛鱼藤和西百灰毛豆的愈伤组织均能合成鱼藤酮，但含量相当低，分别为１７．４μｇ／ｇ，１．８μｇ／ｇ干重。经诱导生根分化的毛鱼藤愈伤组织鱼藤酮含量达１３６．２μｇ／ｇ干重，为愈伤组织含量的７．８倍。西非灰毛豆悬浮培养物的鱼藤酮含量虽比愈伤组织有很大提高，但还不及毛鱼藤愈伤组织含量。结果表明，植物的基因类型在次生代谢上起重要作用，同时离体组织或细胞的分化有助于鱼藤酮的生物合成。

Isolation and HPLC Analysis of Rotenone in In Vitro Tissue Cultures of Plants

Tissues and cells obtained by in vitro culture of Derris elliptica and Tephrosia vogelii were extracted with 9:1 CHCl3/MeOH, purified by gradient filtration of the C --18 reversedphase column and analyzed by HPLC with 66:34 MeOH/H2O or 42:58 CH3CN/H2O as mobile Phase. The results showed that rotenone was biologically synthesized by the callus cultures induced from both species though the content of rotenone was low, 17.4 ug/g and 1.8 ug/g dry weight, respectively. The rotenone content of root--differentiated callus culture of Derris was dramatically increased to 136.2 ug/g dry weight, being 7.8 folds of that of the callus culture. The cell suspension culture of Tephrosia showed higher increase in rotenone content than the tissue culture, but the rotenone content was still lower than that of callus culture of Derris. These results might imply that genotype played an important role in the secondary metabolism and that the dif- ferentiation of tissues or cells favored in vitro biosynthesis of rotenone.