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| 空竹悬浮细胞系的建立 | |
| 引用本文： | 姚娜,岳晋军,顾小平,杨凯,李潞滨.空竹悬浮细胞系的建立[J].核农学报,2011,25(1):43-47. |
| 作者姓名： | 姚娜 岳晋军 顾小平 杨凯 李潞滨 |
| 作者单位： | 1. 中国林业科学研究院林业研究所/国家林业局林木培育重点实验室,北京,100091 2. 中国林业科学研究院亚热带林业研究所,浙江富阳,311400 3. 北京农学院/农业应用新技术北京市重点实验室,北京,102206 |
| 基金项目： | 浙江省重大科技专项重点项目，十一五科技支撑项目，中国林业科学研究院亚热带林业研究所基金，林业公益性行业科研专项 |
| 摘 要： | 竹子悬浮细胞培养比较困难,本研究首次获得空竹悬浮细胞.以空竹种胚为材料诱导愈伤组织,建立稳定的悬浮细胞培养体系;通过固体培养法获得悬浮细胞来源的愈伤组织,可为竹类悬浮细胞系遗传转化或诱变后单克隆的筛选提供材料.空竹种胚在3/4MS+500mg/L脯氨酸+500mg/L谷氨酰胺+300mg/L胰蛋白胨+3.0mg/L 2... |
| 关 键 词： | 空竹 悬浮细胞 细胞培养 |
ESTABLISHMENT OF SUSPENSION CELL LINE OF Cephalostachyum fuchsianum |
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| YAO Na,YUE Jin-jun,GU Xiao-ping,YANG Kai,LI Lu-bin.ESTABLISHMENT OF SUSPENSION CELL LINE OF Cephalostachyum fuchsianum[J].Acta Agriculturae Nucleatae Sinica,2011,25(1):43-47. | |
| Authors: | YAO Na YUE Jin-jun GU Xiao-ping YANG Kai LI Lu-bin |
| Institution: | Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing ; Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang, Zhejiang ; Key Laboratory of Agricultural New Technology and Application in Beijing, Beijing Agricultural College, Beijing |
| Abstract: | Cell suspension culture of bamboo is difficult. The suspension cell line of Cephalostachyum fuchsianum was established for the first time in this study. Seed embryos of Cephalostachyum fuchsianum were used to induce calli. The calli were dedifferentiated from the embyos on the medium of 3/4MS+500mg/L proline+500mg/L glutamine+300mg/L tryptone+3.0mg/L 2,4-D+1.5mg/L KT +30g/L sucrose +5g/L agar. A fine suspension cell line could be established by inoculating the calli in the medium of MS+500mg/L proline+500mg/L glutamine+300mg/L tryptone+1.5mg/L 2,4-D+30g/L sucrose after 3 times shaking subcultures. The subculture cycle was 9d. The calli could be acquired from suspension cell line after the cells being cultured for 25d in the solid medium, and the appropriate inoculation density was 20g/L. |
| Keywords: | Cephalostachyum fuchsianum suspension cells cell culture |
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