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产S-腺苷甲硫氨酸菌株的筛选及发酵工艺优化
引用本文:万荣娥,王启明,张喜团,熊善柏.产S-腺苷甲硫氨酸菌株的筛选及发酵工艺优化[J].华中农业大学学报,2011,30(1):109-114.
作者姓名:万荣娥  王启明  张喜团  熊善柏
作者单位:1. 华中农业大学食品科技学院,武汉,430070
2. 中国科学院微生物研究所,北京,100101
基金项目:中国科学院知识创新青年人才领域前沿项目
摘    要:以45株酵母菌株作为出发菌株,筛选出2株高产SAM(S-腺苷甲硫氨酸)的酿酒酵母菌株(A12A、A18A),以该2株酿酒酵母菌株为对象,研究发酵温度、发酵液初始pH值、摇床转速以及发酵时间对菌体产量和SAM产量的影响,并优化发酵工艺条件,以提高SAM的产量.结果表明:发酵温度、发酵液初始pH值、摇床转速和发酵时间对菌体...

关 键 词:酿酒酵母  S-腺苷甲硫氨酸  发酵工艺
收稿时间:2010/4/17 0:00:00

Screening of Saccharomyces cerevisiae producing S-adenosylmethionine and optimization of fermentation process
WAN Rong-e,WANG Qi-ming,ZHANG Xi-tuan,XIONG Shan-bai.Screening of Saccharomyces cerevisiae producing S-adenosylmethionine and optimization of fermentation process[J].Journal of Huazhong Agricultural University,2011,30(1):109-114.
Authors:WAN Rong-e  WANG Qi-ming  ZHANG Xi-tuan  XIONG Shan-bai
Institution:WAN Rong-e1 WANG Qi-ming2 ZHANG Xi-tuan1 XIONG Shan-bai11.Department of Food Science and Technology,Huazhong Agricultural University,Wuhan 430070 China,2.Institute of Microbiology Chinese Academy of Sciences,Beijing 100101,China
Abstract:The effects of fermentation temperature,the initial pH of fermentation broth,shaking speed and feeding time on the cell dry weight and yield of S-adenosyl-L-methionine(SAM) produced by Saccharomyces cerevisiae(A12A,A18A) selected from 45 yeast strains were studied,and the fermentation conditions was optimized.The results showed that cell dry weight and SAM production were significantly affected(P<0.05) by fermentation temperature,the initial pH,rotation speed and the feeding time.With the orthogonal optimiz...
Keywords:Saccharomyces cerevisiae  S-adenosyl-L-methionine  fermentation process  
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