干旱胁迫下花生苗期耐旱生理应答特性分析

为探明花生响应干旱胁迫的生理机制,以前期试验筛选的花生耐旱品种NH5和HY22,敏感品种FH18和NH16为试材,研究了干旱胁迫下不同花生品种ROS积累与清除能力,保护酶活性、渗透调节物质积累能力以及根系特征,以明确花生应对干旱胁迫的生理应答特性.结果表明,干旱胁迫导致花生的根系萎蔫皱缩且根系活力下降,植株的过氧化氢含...

Physiological response of peanut at seedling stage under drought stress

To deeply understand the physiological mechanism of peanut response to drought stress for breeding and planting in dryland, drought-tolerant peanut varieties NH5 and HY22 and sensitive varieties FH18 and NH16 were selected and used as materials. Reactive oxygen species accumulation and scavenging ability, protective enzyme activity, ability of osmotic regulation substance accumulation and root characteristics were determined to explore physiological response of peanut to drought stress. Results showed that drought stress leads to wilting and activity decreased of root. Hydrogen peroxide accumulated severely in sensitive cultivars and a 2.4 times increase was observed compared to control. Anti-superoxide anion activity was declined significantly in drought sensitive cultivars, while it was slightly affected in drought-tolerant cultivars. The reactive oxygen scavenging system in peanut responded to drought positively, and free proline of each cultivar greatly accumulated under drought stress, with 1.3-4.2 times increase in the 4 cultivars. The soluble protein content increased slightly(between 1.2-2.0 times), and among different cultivars, protective enzyme activity increased by 1.1-5.4 times. The maximum variation coefficient of protective enzyme activity was 22.40% in the drought-tolerant cultivars while 53.36% in sensitive cultivars with low activity. The correlation analysis showed that ascorbate peroxidase was significantly positively correlated with the anti-superoxide anion activity of peanut. In addition, significant positive correlation could be observed between superoxidase dismutase activity and soluble protein, and between peroxidase activity and proline content.