ＢＴ型细胞质雄性不育恢复基因的基因定位

 以典败率、圆败率、染败率、花粉育性和小穗育性为育性指标，对BT型细胞质雄性不育系731A、恢复系C9083以及731A/C9083的F1、731A//731B/C9083的三交F1等亲本和杂种群体的育性进行调查分析，并以731A//731B/C9083的三交F1群体为材料进行RFLP和微卫星标记分析，进行基因定位。结果表明，C9083具有1个主效的显性恢复基因。选用第10染色体上的7个RFLP和微卫星标记分析两个亲本，有3个RFLP标记在两个亲本间有多态；选用其中的C16和G291分析731A//731B/C9083的三交F1中的各个单株的结果表明，这两个RFLP标记与C9083的恢复基因连锁，C16与这个恢复基因之间的交换值为19.3%，G291与这个恢复基因之间的交换值是14.0%， C9083的恢复基因与已报道的BT型细胞质雄性不育恢复基因Rf1可能等位。

Molecular Location of a Fertility Restorer Gene for BT Type CMS Rice

Inheritance of fertility restoration of BT type CMS was conducted by utilizing a CMS line 731A (A), a CMS maintainer line 731B(B), a CMS restorer line C9083(C) and their (A/C)F 1 and (A//B/C)BC 1 populations. The results indicated that fertility restoration of C9083 was conditioned by one dominant gene; the parents C9083 and 731A, and their populations (A//B/C)BC 1 were also used as plant materials to map this gene with RFLP and microsatellite markers on chromosome 10.This gene was linked with RFLP markers C16 and G291, with recombinant value between the gene and C16 being 19.3% and that between the gene and G291 being 14.0%, therefore the gene might be allelic to the Rf1 gene reported before.