防褐化处理对蒙古栎插穗生根及其生理生化的影响

研究防褐化处理对蒙古栎嫩枝扦插生根的影响。以蒙古栎3年实生苗的嫩枝为材料，分别采用乙醇、硝酸银、抗坏血酸对插穗进行防褐化处理，采用500 mg·L^-1 IBA-K浸蘸处理后扦插，并分别在扦插后0、10、20、30、40、50 d取样测定不同生根时期氧化蛋白酶活性和内源激素含量。结果表明，防褐化处理可有效提高扦插生根率，以1%乙醇1 h+500 mg·L^-1 IBA-K处理2 h最佳，生根率、根系效果指数分别为21.1%和5.713。在生根过程中，外施500 mg·L^-1 IBA-K能够提升插穗内POD、PPO的活性，降低IAAO的活性，且IAA/ABA、IAA/ZR的比值升高，有利于蒙古栎嫩枝扦插生根。由此说明1%乙醇1 h浸泡结合500 mg·L^-1 IBA-K激素处理，通过提高POD与PPO的活性，可降低IAAO的活性，增加IAA/ZR和IAA/ABA的比值，提升蒙古栎嫩枝扦插成活率。

Effects of the Anti-Browning Treatment on the Cutting Rooting of Quercus mongolica and Their Physiology and Biochemistry

The effects of the anti-browning treatment on the cutting rooting of Quercus mongolica were studied.The twigs of the 3-year-old seedlings of Q.mongolica were used as materials.Ethanol,silver nitrate,and ascorbic acid were used to conduct the anti-browning treatment,and the cutting were carried out after the dip treatment with 500 mg/L indole-3-butyric acid potassium salt(IBA-K).Meanwhile,samples were taken at 0,10,20,30,40,and 50 days after cutting to determine the oxidative protease activity and endogenous hormone content at different rooting stages.The anti-browning treatment could effectively increase the rooting rate of the cuttings.Among them,the treatment with 1%ethanol for 1h and 500 mg/L IBA-K for 2 h was the best.The rooting rate and the root effect index were 21.1%and 5.713,respectively.During the rooting process,the external application of 500 mg/L IBA-K could increase the activity of POD and PPO in the cuttings,reduce the activity of IAAO,and increase the ratio of IAA/ABA and IAA/ZR,which were beneficial to cuttings.By means of increasing the activity of POD and PPO,dipping with 1%ethanol for 1 h in combination with 500 mg/L IBA-K hormone treatment can reduce the activity of IAAO,increase the ratio of IAA/ZR and IAA/ABA,and improve the cutting survival rate.