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| 利用cDNA-AFLP技术分析苦瓜纯雌系统AgNO_3诱导分化完全花的基因差异表达 | |
| 引用本文： | 王日升,张曼,周生茂,方锋学,杨丽涛,李杨瑞.利用cDNA-AFLP技术分析苦瓜纯雌系统AgNO_3诱导分化完全花的基因差异表达[J].江苏农业学报,2009,25(3). |
| 作者姓名： | 王日升 张曼 周生茂 方锋学 杨丽涛 李杨瑞 |
| 作者单位： | 1. 广西大学农学院,广西,南宁,530005;广西农业科学院蔬菜研究中心,广西,南宁,530007;广西作物遗传改良生物技术重点开放实验室,广西,南宁,530007 2. 广西农业科学院蔬菜研究中心,广西,南宁,530007 3. 广西大学农学院,广西,南宁,530005 4. 广西作物遗传改良生物技术重点开放实验室,广西,南宁,530007 |
| 基金项目： | 国家科技支撑计划项目，公益性行业(农业)科研专项，广西自然科学基金项目，广西作物遗传生物技术重点开放实验室开放课题 |
| 摘 要： | 以苦瓜纯雌系X-黑-d-d为材料,于三叶一心期用300 mg/L AgNO3诱导其分化完全花,并利用cDNA-AFLP技术研究AgNO3 处理后72 h内花蕾基因差异表达情况.结果表明:300 mg/L AgNO3能成功诱导苦瓜纯雌系连续性转化为完全花,平均为11.83朵/株;以128对选择性扩增引物扩增对照和处理后不同时期花蕾的cDNA,其中8对引物可获得较多分布均匀的清晰条带,这些条带大部分是组成型表达,少部分是抑制表达或诱导表达的差异条带. |
| 关 键 词： | 苦瓜 纯雌系 完全花 |
Difference of Genes Expression in the Complete Flowers Induced by AgNO_3 from the Female Flowers of Gynoecious Momordica charantia L. with cDNA-AFLP Technology |
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| WANG Ri-sheng,ZHANG Man,ZHOU Sheng-mao,FANG Feng-xue,YANG Li-tao,LI Yang-rui.Difference of Genes Expression in the Complete Flowers Induced by AgNO_3 from the Female Flowers of Gynoecious Momordica charantia L. with cDNA-AFLP Technology[J].Jiangsu Journal of Agricultural Sciences,2009,25(3). | |
| Authors: | WANG Ri-sheng ZHANG Man ZHOU Sheng-mao FANG Feng-xue YANG Li-tao LI Yang-rui |
| Institution: | 1.College of Agronomy;Guangxi University;Nanning 530005;China;2.Vegetable Research Center;Guangxi Academy of Agricultural Sciences;Nanning 530007;3.Key Open Lab of Guangxi Crop Genetic Improvement and Biotechnology;China |
| Abstract: | The transition of flower was observed after 300 mg/L AgNO3 application on gynoecious line X-Hei-d-d in bitter gourd at three-leaf and one apice stage,and the differential gene expression during bud development after the treatment in 72 h was studied by using cDNA-AFLP technology.The results indicated that 300 mg/L AgNO3 treatment could continuously induce female into complete flowers,in average 11.83 flowers per plant.Eight of 128 pairs of AFLP primer combinations could get evenly distributed and distinct f... |
| Keywords: | AgNO3 cDNA-AFLP |
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