| 日本结缕草愈伤组织的诱导和植株再生 |
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| 引用本文： | 刘拥海,俞乐,崔铁成.日本结缕草愈伤组织的诱导和植株再生[J].草业科学,2010,27(3):107-111. |
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| 作者姓名： | 刘拥海 俞乐 崔铁成 |
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| 作者单位： | 肇庆学院生命科学学院,广东,肇庆,526061;肇庆学院生命科学学院,广东,肇庆,526061;肇庆学院生命科学学院,广东,肇庆,526061 |
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| 基金项目： | 广东省科技计划项目(2008B020300002) |
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| 摘 要： | 以日本结缕草Zoysia japonica Steud的成熟种子为外植体进行了胚性愈伤的诱导与植株再生,结果表明:种子经去皮预处理后,含2.0 mg/L 2,4-D的MS基本培养基能成功诱导去皮种子产生愈伤组织,愈伤组织诱导率高达87.1%。愈伤组织在继代过程中共产生4种不同的类型。1/2 MS中添加0.4 mg/L KT或添加1.0 mg/L KT和1.0 mg/L NAA均能有效促进胚性愈伤的分化,分化率分别为63.8%、66.0%。
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| 关 键 词： | 日本结缕草 愈伤诱导 植株再生 |
Study on callus induction and plant regeneration of zoysiagrass |
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| LIU Yong-hai,YU Le,CUI Tie-cheng.Study on callus induction and plant regeneration of zoysiagrass[J].Pratacultural Science,2010,27(3):107-111. |
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| Authors: | LIU Yong-hai YU Le CUI Tie-cheng |
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| Institution: | Life Sciences College;ZhaoQing University;Guangdong ZhaoQing 526061;China |
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| Abstract: | In order to achieve embryogenic callus induction and plant regeneration,the sterilized mature seeds of zoysiagrass(Zoysia japonica Stend.) were used as explants.The results indicated that peeled seeds after pretreatment in MS containing 2.0 mg/L 2,4-D could successfully induce callus,the frequency was as high as 87.1%.In the course of subculture,there were four different types of callus.1/2 MS containing 0.4 mg/L KT,or 1/2 MS containing 1.0 mg/L KT and 1.0 mg/L NAA was able to promote the differentiation of... |
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| Keywords: | zoysiagrass (Zoysia japonica Stend) callus induction plant regeneration |
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