| 华山松胚性愈伤组织的诱导研究 |
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| 引用本文： | 逯昀,孙景梅,侯佳.华山松胚性愈伤组织的诱导研究[J].北方园艺,2010(11):155-157. |
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| 作者姓名： | 逯昀 孙景梅 侯佳 |
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| 作者单位： | 商丘职业技术学院,园林食品加工系,河南,商丘,476000;商丘职业技术学院,园林食品加工系,河南,商丘,476000;西南林学院,资源学院,云南,昆明,650224;西南林学院,资源学院,云南,昆明,650224 |
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| 摘 要： | 以华山松的成熟胚为试材,采用正交实验设计方法,研究培养基类型及所需激素浓度对胚性愈伤组织诱导的影响,寻求其形成条件。结果表明:培养基成分为LM+2,4-D 2 mg/L+BA 2 mg/L+CH 1000 mg/L+肌醇1000 mg/L+Gln 500 mg/L可诱导华山松成熟胚愈伤组织的形成,其中类似胚性愈伤组织的诱导率达60%。培养30 d后将愈伤组织转接至与诱导培养基组分相同的新鲜培养基,可观察到愈伤组织生长良好并发生体积上的扩增,但没发生分化。
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| 关 键 词： | 华山松 成熟胚 组织培养 |
Induced Research on the Embryos Callus Tissue of Pinus armandii |
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| LU Yun,SUN Jing-mei,HOU Jia.Induced Research on the Embryos Callus Tissue of Pinus armandii[J].Northern Horticulture,2010(11):155-157. |
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| Authors: | LU Yun SUN Jing-mei HOU Jia |
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| Institution: | LU Yun1,SUN Jing-mei1,2,HOU Jia2 (1.Shang qiu Vocational and Technical College,Gardening and Food Processing Department,Shangqiu,Henan,476100,2.Institute of Resources,Southwest Forestry University,Kunming,Yunnan 650224) |
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| Abstract: | This experiment by the mature embryo of Pinus armandii to the material,with the aid of the orthogonal test design method,to research the influence of culture-medium types and the hormone density of the needing to the injuriy which the embryo-organization sinduced seeked the condition which it formed.The results indicated the culture medium ingredient LM+2,4-D 2 mg/L+BA 2 mg/L+hydrolisis casein 1 000 mg/L+phaseomannite l 000 mg/L+Gln 500 mg/L might induce the injury of the mature embryo of Pinus armandii,the... |
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| Keywords: | Pinus armandii the mature embryo tissue culture |
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