成熟期短时渍水胁迫对烤烟碳氮代谢关键酶及主要碳、氮化合物含量的影响

以烤烟云烟97为材料,采用田间试验研究了成熟期短时渍水处理对烤烟碳氮代谢关键酶活性及主要碳、氮化合物含量的影响。结果表明,在烤烟成熟期渍水处理3 d,中部叶片硝酸还原酶活性低于对照,降幅为20.39%;转化酶和淀粉酶活性高于对照,增幅分别为33.14%,56.70%;烟叶淀粉、总氮和烟碱含量有降低趋势,分别下降了2.9,0.74,0.77个百分点,而总糖和还原糖含量有增加趋势,分别上升了1.33,0.46个百分点。说明渍水胁迫使叶片生理代谢紊乱,氮代谢减弱,碳水化合物积累减少。研究结果有助于从生理生化角度揭示成熟期渍水胁迫对烤烟生长和品质形成影响的机制。

The Influence on the Key Enzymes of Carbon-nitrogen Metabolism and the Content of the Main Carbonitride at the Maturity of Transitorily Waterlogged Flue-cured Tobacco

A field experiment was conducted to study the effect of transient waterlogging stress on the activity of carbon-nitrogen metabolism key enzymes and the content of the main carbonitride of flue-cured tobacco CV.Yunyan97 during maturing stage.The results indicated that in the middle leaves of waterlogged flue-cured tobacco at the maturity,after waterlogging treatment for three days,the nitrate reductase activity with the decreased range 20.39% was lower,the invertase and amylase activity with the increased range 33.14% and 56.70% respectively was higher,compared with that in control treatment;the content of starch,total nitrogen and nicotine decreased by 2.90 percent,0.74 percent and 0.77 percent separately,but the content of total sugar and reducing sugar respectively increased by 1.33 percent and 0.46 percent.The above results showed that the physiological metabolism of flue-cured tobacco at the maturity was disordered,nitrogen metabolism wakened,and the accumulation of carbohydrate decreased under waterlogging stress.The conclusion of the study was benefiticial to reveal the mechanism of influence on the growth and quality of mature flue-cured tobacco under waterlogging stress from the physiological and biochemical perspective.