| 鸡毒支原体F弱毒疫苗株细胞免疫特性研究 |
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| 引用本文： | 林伯全,徐磊,杨慧,林秋敏.鸡毒支原体F弱毒疫苗株细胞免疫特性研究[J].中国动物传染病学报,2014(3):78-81. |
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| 作者姓名： | 林伯全 徐磊 杨慧 林秋敏 |
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| 作者单位： | 福建农业职业技术学院动物科学系 |
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| 基金项目： | 新世纪“百千万人才工程”国家级人选补助基金项目(NCNCMTPC-2009);2011年福建省教育厅A类科技项目(JA11320);2012年福州市科技计划项目(2012-N-94) |
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| 摘 要： | 为了解鸡毒支原体（Mycoplasma gallisepticum,MG）F弱毒疫苗株的细胞免疫特性,分别以活菌浓度为109（A组）、106（B组）ccu/mL的MG F株及生理盐水（C组）点眼接种SPF鸡,采用流式细胞技术及T淋巴细胞增殖试验对免疫前后淋巴细胞亚类Th/T、Tc/T、Th/Tc及刺激指数（SI）的动态变化规律进行研究。结果显示免疫后A、B组的Th/T、Tc/T、SI明显升高,其中Th/T于d5、d7,Tc/T、SI于d7、d14,A组显著高于B组（P〈0.05）。研究结果表明,免疫MG F株可较好的提高鸡的细胞免疫,且MG F株活菌浓度与接种鸡外周血Th/T、Tc/T、SI呈正相关。
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| 关 键 词： | 鸡毒支原体 F弱毒疫苗 细胞免疫 淋巴细胞亚类 淋巴细胞增殖 |
THE EFFECT OF MYCOPLASMA GALLISEPTICUM F VACCINE ON CELLULAR IMMUNITY |
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| LIN Bo-quan;XU Lei;YANG Hui;LIN Qiu-min.THE EFFECT OF MYCOPLASMA GALLISEPTICUM F VACCINE ON CELLULAR IMMUNITY[J].Chinese Journal of Animal Infectious Diseases,2014(3):78-81. |
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| Authors: | LIN Bo-quan;XU Lei;YANG Hui;LIN Qiu-min |
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| Institution: | LIN Bo-quan;XU Lei;YANG Hui;LIN Qiu-min;Department of Animal Science, Fujian Agricultural Vocational Technical College; |
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| Abstract: | To evaluate the effect of Mycoplasma gallisepticum （MG） F vaccine on cellular immunity, SPF chickens were inoculated with 109 ccu/mL MG （A group）, 106 ccu/mL MG （B group） and saline （C group）, respectively. In addition, the change of Th/T, Tc/T, Th/Tc and SI were analyzed by Flow cytometry and lymphocytes proliferation test. The results showed that Th/T, Tc/T, Th/Tc and SI of A and B group increased significantly. Both Th/T on 5 and 7 days post vaccination （dpv） and Tc/T, SI on 7 and 14 dpv of A group were significantly higher than B group （P〈0.05）. These findings indicated that MG F vaccine was able to enhance the cellular immune response and Th/T, Tc/T and SI in peripheral blood mononuclear cells （PBMCs） were positively correlated with the inoculation close of MG F vaccine. |
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| Keywords: | Mycoplasma gallisepticum （MG） F attenuated vaccine cellular immunity lymphocyte subpopulation lymphocytes proliferation |
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