| 极度高甘油三脂高胆固醇血症动物模型的建立 |
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| 引用本文： | 贺亮,李锐,邓旭明.极度高甘油三脂高胆固醇血症动物模型的建立[J].东北农业大学学报,2009,40(2). |
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| 作者姓名： | 贺亮 李锐 邓旭明 |
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| 作者单位： | 1. 吉林大学畜牧兽医学院,长春,130062
2. 浙江省农业科学院,杭州31002D |
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| 摘 要： | 建立高脂血症以及联合高脂血症和糖尿病的金黄地鼠模型,为深入研究高脂症在动脉粥样硬化和糖尿病合并动脉粥样硬化等疾病中的作用提供耗时短、效果稳定的动物模型。试验组金黄地鼠(90～110 g)连续3 d腹腔注射小剂量链脲佐菌素(STZ)(30 mg.kg-1)。于注射后9 d,检测空腹血糖,选择血糖大于200 mg.dL-1的个体,给予高脂饲料(HF),对照组同时给予普通饲料(chow)或高脂饲料(HF),检测4周血浆葡萄糖(Glu)、甘油三酯(TG)、总胆固醇(TC)等指标。结果表明,地鼠注射STZ后血糖升高并维持在300～400 mg·dL-1。在喂饲高脂饲料4周后,糖尿病高脂组血脂极度升高,TG达20 000 mg·dL-1,TC达4 500 mg·dL-1;不注射STZ组饲喂高脂饲料后TG和TC也明显升高至1 000 mg·dL-1左右。利用高脂饲喂或联合STZ注射的方法,可以成功制造出稳定的高脂血症以及联合糖尿病的金黄地鼠模型。
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| 关 键 词： | 金黄地鼠 高脂血症 动物模型 |
Development of hyperlipidemia animal model |
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| HE Liang,LI Rui,DENG Xuming.Development of hyperlipidemia animal model[J].Journal of Northeast Agricultural University,2009,40(2). |
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| Authors: | HE Liang LI Rui DENG Xuming |
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| Institution: | HE Liang1,LI Rui2,DENG Xuming1(1.College of Animal Science , Veterinary Medicine,Jilin University,Changchun 130062,China,2.Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China) |
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| Abstract: | The objective of the present study is to develop a quick and stable golden hamster model of hyperlipidemia and a model of diabetes combined with hyperlipidemia,which is suitable for studying the role of hyperlipidemia in artherosclerosis.Male hamsters(90-110 g) were divided into two groups and injected intraperi-toneally with low dose of streptozotocin(STZ)(30 mg.kg-1) or vehicle only,9 d after injection,hamsters which had plasma glucose higher than 200 mg.dL-1 in STZ group were selected and fed on high-fat... |
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| Keywords: | golden hamster hyperlipidemia animal model |
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