大豆对SMV株系SC10的抗性遗传及抗病基因的定位研究

【目的】明确大豆对SMV株系SC10的抗性遗传方式以及不同抗源所携带的抗病基因间的等位关系,并对科丰1号所携带的抗SC10基因进行标记定位。【方法】利用抗中国南方大豆产区流行株系SC10的科丰1号、晋大74、大白麻、汾豆56、中作229、徐豆1号、邳县茶豆、Kwanggyo、跃进4号配制部分抗抗杂交组合并与感病品种南农1138-2和8101配制抗感组合,在接种SC10的条件下,调查各组合后代的抗感反应;并利用科丰1号×南农1138-2的F2群体和分离群体分组分析法（BSA）及SSR标记对抗病基因进行标记定位。【结果】科丰1号、晋大74、大白麻、汾豆56、中作229和徐豆1号与感病品种杂交的F1表现抗病,F2呈3抗﹕1感分离比例,F2:3家系呈1抗﹕2分离﹕1感病的分离比率;晋大74×汾豆56、徐豆1号×邳县茶豆的F1表现抗病、F2未发现感病株。科丰1号×Kwanggyo、汾豆56、中作229,晋大74×中作229、Kwanggyo,大白麻×汾豆56、科丰1号和跃进4号×Kwanggyo的F1表现抗病,F2呈15抗﹕1感的分离比例,F2:3家系符合7抗﹕4分离（15抗﹕1感）﹕4分离（3抗﹕1感）﹕1感的分离比例;D1b连锁群上的SSR标记Satt558、Sat₂54、Satt634、Gm020580、Gm020584、Gm020562和Gm020546与抗病基因RSC10连锁,遗传距离分别为6.1、2.0、0.9、0.8、2.0、4.6和9.2 cM。【结论】科丰1号、晋大74、大白麻、徐豆1号、汾豆56、中作229各有一个显性基因控制对SC10株系的抗性;晋大74与汾豆56,徐豆1号与邳县茶豆的抗病基因是等位的或紧密连锁的;科丰1号与Kwanggyo、汾豆56、中作229携带的抗SC10株系的基因处于不同位点,晋大74与中作229、Kwanggyo携带的抗SC10株系的基因处于不同位点,大白麻与汾豆56、科丰1号携带的抗SC10株系的基因处于不同位点,跃进4号与Kwanggyo携带的抗SC10株系的基因处于不同位点;科丰1号对SC10株系的抗病基因RSC10位于D1b连锁群。

Studies on Mapping and Inheritance of Resistance Genes to SMV Strain SC10 in Soybean

【Objective】The inheritance of resistance and allelism of resistance genes to SMV strain SC10 was studied and the resistance genes to SC10 in Kefeng No.1 were mapped.【Method】The resistant soybean cultivars to SMV strain SC10(Kefeng No.1, Jinda74, Dabaima, Fendou56, Zhongzuo229, Xudou No.1, Pixianchadou, Kwanggyo and Yuejin No.4)were crossed with susceptible (S) cultivars (Nannong 1138-2 or 8101), respectively, to determine the inheritance of resistance to SC10. Each R parents were also crossed with each other to test the allelism of the resistance genes. The bulked segregant analysis (BSA) of SSR markers and F2 population from Kefeng No.1 × Nannong 1138-2 were used in mapping the resistance gene.【Result】The F1 of crosses from resistant cultivars (Kefeng No.1, Jinda74, Dabaima, Fendou56, Zhongzuo229, Xudou No.1) and susceptible (S) cultivars (Nannong 1138-2 or 8101) were resistant, F2 segregated in 3 resistant﹕1 susceptible ratio, and 1R﹕2Seg﹕1S in F2:3 families. The F1 and F2 populations from Jinda74×Fendou56 and Xudou No.1×Pixianchadou exhibited complete resistant. F1 from the two crosses of Kefeng No.1×Kwanggyo, Fendou56 and Zhongzuo229, Jinda74×Zhongzuo229 and Kwanggyo, Dabaima×Fendou56 and Kefeng No.1. Yuejin No.4×Kwanggyo were all resistant while their correspond F2 population segregated in a ratio of 15(R)﹕1(S). The seven SSR markers (Satt558, Sat_254, Satt634, Gm020580, Gm020584, Gm020562 and Gm020546) were found linking to RSC10. The genetic distance between the resistance gene RSC10 and SSR markes were 6.1, 2.0, 0.9, 0.8, 2.0, 4.6, and 9.2 cM, respectively.【Conclusion】 Kefeng No.1, Jinda74, Dabaima, Xudou No.1, Fendou 56, Zhongzuo229, respectively, have a single dominant gene controlling the resistance to SC10. The resistance genes in Jinda74 and Fendou 56, Xudou No.1 and Pixianchadou were at a same locus or very closely linked. It was inferred that the resistance genes between Kefeng No.1 and Kwanggyo, Fendou56, Zhongzuo229; between Jinda74 and Zhongzuo229, Kwanggyo; between Dabaima and Fendou56, Kefeng No.1; between Yuejin No.4 and Kwanggyo were not at the same locus. The resistance gene carried by Kefeng No.1 to SC10 was located on D1b linkage group.