花生萌发期对低温胁迫的生理生化响应机制

为探讨不同花生品种低温胁迫下的萌发差异成因,为花生耐低温种植及抗冷性研究提供理论依据,本研究以耐低温品种豫花22号和中间耐性品种漯花16号以及低温敏感型品种泉花6号、鲁花11号为材料,测定不同温度处理条件下萌发相关的生理指标.结果显示,与CK相比,低温处理显著降低了各花生品种的露白率、发芽率、相对发芽率,不同抗性品种存...

Physiological and biochemical response mechanism of peanut germination to low temperature stress

To explore the germination differences of different peanut varieties under low temperature stress, and provide a theoretical basis for low temperature tolerance and cold resistance of peanut, low temperature tolerant varieties Yuhua 22 and intermediate varieties Luohua 16, low-temperature sensitive varieties Quanhua 6 and Luhua 11 were used as experimental materials to determine the physiological indexes related to germination under cold treatment. The results showed that, compared with CK, low temperature treatment 2℃ significantly reduced the radicle emergence rate, germination rate and relative germination rate of each peanut variety, and there were significant differences among the varieties. The decline reats of low temperature-tolerant peanut varieties were less than 10%, while that of intermediate and sensitive varieties was 30% to 100%. Under 2℃ stress for 24 h, the electrical conductivity of sensitive varieties was significantly higher than that of low temperature resistant and intermediate varieties. With the extension of stress time, SOD, POD activity increased, CAT activity decreased. Under 2℃ stress for 48 h, the content of Ss （soluble sugar） was higher than CK, the content of SP （soluble protein） was lower than CK. MDA content of sensitive varieties increased significantly, while that of low-temperature tolerant varieties increased slightly. The SOD activity of low-temperature-tolerant varieties was higher than that of intermediate and sensitive varieties, and the CAT activity decreased more significantly than that of intermediate and sensitive varieties. During recovery period, MDA content of all varieties was lower than CK, SP accumulation was higher than CK, Ss accumulation of low temperature resistant varieties and intermediate varieties was higher than sensitive varieties. The activities of antioxidant enzymes SOD, POD and CAT showed the same trend. With the extension of germination time, the enzyme activity of peanut varieties with stronger cold resistance was higher. The differences in each index well reflected the differences in low temperature tolerance of different resistant peanut varieties. The results of this study provide a basis for further improving the agronomic measures to prevent chilling injury in peanut and conducting peanut breeding for low temperature tolerance.