| 大豆荚皮厚性状QTL定位及候选基因挖掘 |
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| 引用本文： | 王乔,蒋洪蔚,谢建国,潘文婧,郑海洋,侯立龙,熊心,武小霞. 大豆荚皮厚性状QTL定位及候选基因挖掘[J]. 中国油料作物学报, 2022, 44(2): 298-306. DOI: 10.19802/j.issn.1007-9084.2021061 |
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| 作者姓名： | 王乔 蒋洪蔚 谢建国 潘文婧 郑海洋 侯立龙 熊心 武小霞 |
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| 作者单位： | 1.东北农业大学，黑龙江 哈尔滨，1500302.吉林省农业科学院, 吉林省 长春市 邮编 130033 |
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| 基金项目： | 国家自然科学基金(31771882);黑龙江省自然科学基金(C2018017);国家重点研发计划大豆项目(2016YFD0100201-24);黑龙江省农业科技创新跨越工程大豆项目(HNK2019CX01-11) |
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| 摘 要： | 大豆(Glycine max L.)的荚皮厚是影响大豆产量的重要因素,同时,大豆荚皮内含有丰富的蛋白质和纤维素等营养物质,是植物饲料的重要来源.因此,大豆荚皮厚性状的相关研究,对大豆的专用品种育种以及大豆深加工都具有重要意义.本研究利用ICIM法对CSSLs群体进行QTL定位,同时构建高低混池,利用ICIMapping...
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| 关 键 词： | 荚皮厚 QTL定位 候选基因 |
| 收稿时间： | 2021-02-24 |
QTL mapping and candidate gene mining for pod thickness in soybean |
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| Qiao WANG,Hong-wei JIANG,Jian-guo XIE,Wen-jing PAN,Hai-yang ZHENG,Li-long HOU,Xin XIONG,Xiao-xia WU. QTL mapping and candidate gene mining for pod thickness in soybean[J]. Chinese Journal of Oil Crop Sciences, 2022, 44(2): 298-306. DOI: 10.19802/j.issn.1007-9084.2021061 |
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| Authors: | Qiao WANG Hong-wei JIANG Jian-guo XIE Wen-jing PAN Hai-yang ZHENG Li-long HOU Xin XIONG Xiao-xia WU |
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| Affiliation: | 1.Northeast Agricultural University, Harbin, Heilongjiang 150030, China2.Jilin Academy of Agricultural Sciences, Changchun, Jilin 130033, China |
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| Abstract: | The pod thickness of soybean (Glycine max L.) is an important factor affecting the yield of soybean. Soybean pod is also rich in protein, cellulose and other nutrients, which can be used as stockfeed. Therefore, the research on pod thickness is of great significance to the special variety breeding of soybean and the further processing of soybean. In this study, ICIM method was used to locate QTL in CSSLs population, and a high-low mixed pool was constructed. BSA was used to analyze the re-sequencing results. Finally, the confidence interval was reduced to 0.03 Mb (2 970 674 bp-3 000 004 bp) on chromosome 03. All the genes in the consensus QTL interval were annotated, and the four candidate genes were obtained. Through gene annotation information and related biological analysis, two target genes directly related to pod thickness trait were predicted. The results of this study will have a certain reference value for soybean fine breeding. |
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| Keywords: | pod thickness QTL mapping candidate genes |
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