| 大豆三粒荚数相关QTL的分子标记开发与验证 |
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| 引用本文： | 侯立龙,蒋洪蔚,熊心,郑海洋,曹富斌,魏茹茹,张一超,赵雨轩,郭梦瑶,陈庆山. 大豆三粒荚数相关QTL的分子标记开发与验证[J]. 中国油料作物学报, 2022, 44(6): 1239-1248. DOI: 10.19802/j.issn.1007-9084.2021275 |
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| 作者姓名： | 侯立龙 蒋洪蔚 熊心 郑海洋 曹富斌 魏茹茹 张一超 赵雨轩 郭梦瑶 陈庆山 |
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| 作者单位： | 1.东北农业大学，黑龙江 哈尔滨，1500302.吉林省农业科学院，吉林 长春，130033 |
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| 基金项目： | 国家自然科学基金区域联合重点项目(U20A2027);国家自然科学基金(31771882);2019年吉林省科研院所引进高层次科研创新人才资助计划 |
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| 摘 要： | 本研究基于大豆三粒荚数相关QTL开发InDel标记，并利用自然群体和育种群体分别对InDel标记进行多态性的筛选和应用效果的验证，以期实现分子标记辅助选择，最后利用大豆三粒荚数相关QTL内的候选基因表达情况验证QTL的有效性。经过4个育种群体验证，引物INDEL3-311497、INDEL17-440117和INDEL17-462053可用于加拿大蛋白豆与吉育4512杂交群体的验证，INDEL13-240031可用于齐农7号与辛大粒杂交群体中的验证，INDEL3-250092、IN-DEL3-311497和INDEL13-254396可用于东富豆8号与通试豆4杂交群体中的验证,INDEL3-240669和INDEL13-246066可用于吉育441与合丰55杂交群体中的验证。表达量分析结果表明，Glyma.03G029800和Glyma.17G062600两个候选基因促进大豆三粒荚形成及发育...
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| 关 键 词： | 大豆 三粒荚数 分子标记 标记辅助选择 |
| 收稿时间： | 2021-10-31 |
Development and verification of molecular markers for QTLs related to the number of three-seeded pods in soybean |
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| Li-long HOU,Hong-wei JIANG,Xin XIONG,Hai-yang ZHENG,Fu-bin CAO,Ru-ru WEI,Yi-chao ZHANG,Yu-xuan ZHAO,Meng-yao GUO,Qing-shan CHEN. Development and verification of molecular markers for QTLs related to the number of three-seeded pods in soybean[J]. Chinese Journal of Oil Crop Sciences, 2022, 44(6): 1239-1248. DOI: 10.19802/j.issn.1007-9084.2021275 |
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| Authors: | Li-long HOU Hong-wei JIANG Xin XIONG Hai-yang ZHENG Fu-bin CAO Ru-ru WEI Yi-chao ZHANG Yu-xuan ZHAO Meng-yao GUO Qing-shan CHEN |
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| Affiliation: | 1.Northeast Agricultural University, Harbin 150030, China2.Jilin Academy of Agricultural Sciences, Changchun, 130033, China |
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| Abstract: | To realize the purpose of marker-assisted selection, InDel markers were developed based on QTL for three-seeded pod number of soybeans, the natural population and breeding population were used to screen the polymorphism of InDel markers and verify the application effect, respectively. Finally, the expression of candidate genes in QTL for three seed-pod number was used to verify the validity of QTL. The results showed that primers INDEL3-311497, INDEL17-440117 and INDEL17-462053 could be employed to select the progeny in the hybrid population of Canadian Danbaidou and Jiyu 4512, INDEL13-24003 could be used in the hybrid population of Qinong 7 and Xindali, INDEL3-250092, INDEL3-311497 and INDEL13-254396 could be used in the hybrid population between Dongfudou 8 and Tongxiangdou 4, INDEL3-240669 and INDEL13-246066 could be used for progeny selection in the hybrid population of Jiyu 441 and Hefeng 55. Two candidate genes, Glyma.03G029800 and Glyma.17G062600, promoted the formation and development of three-seeded pods, while two candidate genes Glyma.17G062000 and Glyma.13G063700 inhibit the formation and development of three-seeded pods. These results indicated that the four QTL loci obtained in our laboratory may be related to the formation of three soybean pods, which also confirmed the effectiveness of InDel markers on breeding population selection. |
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| Keywords: | soybean number of three-seeded pods molecular marker marker-assisted selection |
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