Aluminum-induced oxidative stress in lymphocytes of common carp (<Emphasis Type="Italic">Cyprinus carpio</Emphasis>) |
| |
Authors: | Sandra García-Medina Amparo Celene Razo-Estrada Leobardo Manuel Gómez-Oliván Araceli Amaya-Chávez Eduardo Madrigal-Bujaidar Marcela Galar-Martínez |
| |
Institution: | 1.Escuela Nacional de Ciencias Biológicas, Laboratorio de Toxicología Acuática, Departamento de Farmacia,Instituto Politécnico Nacional,Mexico,Mexico;2.Escuela Nacional de Ciencias Biológicas, Laboratorio de Genética, Sección de Estudios de Posgrado e Investigación,Instituto Politécnico Nacional,Mexico,Mexico;3.Facultad de Química, Departamento de Farmacia,Universidad Autónoma del Estado de México,Toluca,Mexico |
| |
Abstract: | Several studies of fish have shown that aluminum may induce hypoxia, hypercapnia, metabolic acidosis, and respiratory failure.
In lymphocytes, morphologic abnormalities and reduced immune activity have been observed. Nevertheless, there is little data
on oxidative stress and such data are essential in order to identify its mechanism of action. The common carp Cyprinus carpio, an omnivorous fish commonly used in commercial aquaculture, has been proposed as a test organism in toxicologic assays due
to its economic importance and wide geographic distribution. The aim of this work was to evaluate Al-induced oxidative stress
in lymphocytes of the common carp C. carpio. Specimens were exposed to three different concentrations of Al (0.05, 120, and 239 mg/l) in a static exposure system for
96 h. At the end of the exposure period, blood was collected and lymphocytes were separated. Lipid peroxidation, oxidized
protein content and the activity of superoxide dismutase, catalase, and glutathione peroxidase were measured. Results show
that the tested Al concentrations modified the activity of antioxidant enzymes and elicited higher levels of lipid peroxidation
and oxidized proteins. The degree of damage induced was concentration and tissue dependent. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|