枯草芽孢杆菌和苏云金芽孢杆菌的共培养及其对生物活性物质产生的影响

研究了枯草芽孢杆菌Bs5 5和Bs90菌株分别与苏云金芽孢杆菌Bt1和Bt2菌株在共培养条件下的菌体生长、抗菌活性物质脂肽类化合物和杀虫活性物质伴孢晶体蛋白的产生状况。结果表明 ,Bs5 5与Bt共培养的菌体生长和脂肽类化合物的产生与单独培养的Bs5 5相似 ,未检测到伴孢晶体蛋白 ,但有Bt特异性蛋白存在 ;Bs90与Bt共培养的菌体生长和脂肽类化合物的产生与单独培养的Bs90有相对较大的差别 ,检测到伴孢晶体蛋白。说明Bs与Bt可以共生 ,组合适当时可以产生各自的生物活性物质

Co-culture of Bacillus subtilis and Bacillus thuringiensis and its influence on production of bioactive substances

The bacterial growth,production of antimicrobial lipopeptides and toxic parasporal crystal protein were studied under the condition of combination of Bacillus subtilis Bs55 and Bs90 strains with Bacillus thuringiensis Bt1 and Bt2 strains.The results showed that the bacterial growth and the production of lipopeptide in combination of Bs55 with Bt strains were similar to Bs55 alone and parasporal crystal protein was not detected but Bt characteristic protein was found.While bacterial growth and the lipopeptide production in combination of Bs90 with Bt strains were relatively different from Bs90,and crystal protein was detected.The results indicate that Bacillus subtilis and Bacillus thuringiensis can be co grown,and can produce their bioactive substances in a suitable combination.