水稻RFLP遗传作图群体重建的初步研究

用已定位的94个RFLP标记为探针,测定了12种限制性内切酶酶切后的F_2群体中58个单株及其双亲的RFLP表现。根据各标记F_2群体中的连锁分析资料,初步重建了水稻RFLP遗传作图群体。结果表明:51个标记被定位在10条染色体上,它们的连锁群和定位位置相同于原遗传图;18个标记揭示了双亲间的多态性,但是,没有发现与其它标记的连锁关系;12个标记至少在1种酶切条件下揭示出RFLP,然而,它们现属的连锁群有别于原遗传图;其余13个标记无法揭示RFLP存在。这一遗传群体可供新的DNA标记的定位。

RECONSTRUCTION OF RFLP MAPPING POPULATION IN RICE(Oryza sativa L.)

A transformation of RFLP mapping population was constructed. 94 genomic DNA markers which mapped on rice genetic map were used as probes to detect RFLP pattern of F_2 population plant and their parents which were digested by 12 restriction endonucleases. Following linkage analysis of these markers in F_2 population, 51 markers were mapped on 10 chromosomes, their linkage groups and location order were similar to previous reported maps; 18 markers revealed polymorphism between the parents, but were found no linkage with any marker, 12 markers detected difference with at least one of the 12 restriction enzyme digest tested; however the linkage group was different from the previous RFLP map, the other 13 markers were monomorphism between the parents and were not investigated further. This population can be used to map new DNA markers.