花生幼苗不同切断及其愈伤组织的农杆菌介导转化

试验进行了不同花生外植体（成熟胚的幼叶、胚轴、子叶等）的直接农杆菌转化与花生愈伤组织的农杆菌转化,比较其感染后的诱芽与上分化生长情况,并对影响农杆菌介导转化花生的因素进行了探讨.结果表明农杆菌的生长状况、浓度及侵染时间和筛选剂的浓度是影响农杆菌介导花生转化的主要因素;褐化是影响其转化的关键问题.

Peanut Genetic Transformation by Agrobacterium Tumer ficiences

Studied Agrobacterium-mediated transformation on peanut explants.First,we affected different explants with A.T,including leave,stem,cotyledon and axillary bud,which were just cut from healthy seedlings.Second,after in- duced callus from there different explants,we affected callus with A.T.Finally,we also discussed the factors of this ap- proach.The results showned explants could still produce healthy callus after A.T affection;the main reason caused failure of two kind of transformation is brownlization;Peanut was more easy to brownlize than other cereal,such as maize and wheat;different explants had no difference with the same cb concentration,and the appropriate conditions were as the following:500mg/L cb,100uL/L PPT,no more than 5minutes affection with A.T,but different explants had light dif- ferent responsibilitiea.In sum,the key points affecting result of peanut Agrobacter/um-mediated transformation was A.T, affected concemration and PPT selected concentration.