Identification of sex in Carica papaya L. using RAPD markers |
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Authors: | Eliana Gertrudes Macedo Lemos Cristina Lacerda Soares Petrarolha Silva Humberto Actis Zaidan |
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Institution: | 1. Departamento de Tecnologia, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, Campus Jaboticabal, Via de Acesso Prof. Paulo Donato Castellane, s/n, km 5, 14884-900, Jaboticabal, S?o Paulo, Brasil
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Abstract: | Brazil is currently the worlds largest producer of papaya (Carica papayaL.), producing fruits for both the domestic market and export. Only fruits from hermaphrodite plants are marketed because
they have the necessary commercial characteristics, i.e. they are pear-shaped and have thicker flesh and a smaller internal
cavity. Increased papaya yield has been limited mainly by the ratio of female to hermaphrodite (1: 2) plants normally occurring
in orchards. This ratio causes great losses to papaya producers and the identification of the sex of seedlings during the
nursery stage would be an important advance. In our study random amplified polymorphic DNA (RAPD) analysis was used to differentiate
between the sexual forms of three commercial C. papaya cultivars belonging to the Solo group. RAPD assays using the BC210 primer were able to detect hermaphrodites in all of the
cultivars tested. The BC210438molecular marker was much better at papaya sex differentiation than other markers described in the literature.
This revised version was published online in August 2006 with corrections to the Cover Date. |
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Keywords: | Carica papaya hermaphrodite RAPD sexdetermination sex-linked markers |
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