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我国桃树新病害——叶枯病的病原菌鉴定及生物学特性研究
引用本文:张勇,李晓军,曲健禄,范昆,杨建明.我国桃树新病害——叶枯病的病原菌鉴定及生物学特性研究[J].园艺学报,2010,37(11):1745-1750.
作者姓名:张勇  李晓军  曲健禄  范昆  杨建明
作者单位:(山东省果树研究所,山东泰安 271000)
基金项目:国家科技支撑计划项目,国家科技基础条件平台项目
摘    要:对我国桃树上发生的一种新病害进行了病原鉴定及生物学特性研究。通过形态特征观察、致病性测定、rDNA-ITS序列测定及分析,确定该病害为立枯丝核菌(Rhizoctonia solaniK ühn)引起的叶枯病,病原菌rDNA-ITS区序列已在GenBank上登录(GenBank登录号:GU270581)。病原菌生长的适宜温度为25~30℃,适宜pH5~7,适宜碳源为蔗糖,适宜氮源为酵母。

关 键 词:桃树  叶枯病  立枯丝核菌  生物学特性

Pathogen Identification and Biological Characteristic of Leaf Blight——A New Peach Disease in China
ZHANG Yong,LI Xiao-jun,QU Jian-lu,FAN Kun,YANG Jian-ming.Pathogen Identification and Biological Characteristic of Leaf Blight——A New Peach Disease in China[J].Acta Horticulturae Sinica,2010,37(11):1745-1750.
Authors:ZHANG Yong  LI Xiao-jun  QU Jian-lu  FAN Kun  YANG Jian-ming
Institution:(Shandong Institute of Pomology,Tai’an,Shandong 271000,China)
Abstract:The pathogen causing leaf blight on peach was isolated from diseased leaf. Based on morphological characteristics,pathogenicity test,the sequence of ribosomal DNA-ITS,the pathogen was identified as Rhizoctonia solani Kühn,the rDNA-ITS sequence data of the pathogenic fungus has been submitted to GenBank(GenBank accession number:GU270581). The temperature for mycelial growth of the pathogen ranged from 10 ℃ to 35 ℃,with the optimum temperature 25–30 ℃. The pH ranged from 4–10 with the optimum pH 5–7. For mycelial growth,the optimum carbon and nitrogen source were sucrose and yeast,respectively.
Keywords:peach  leaf blight  Rhizoctonia solani Kühn  biological characteristics
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