15个樱桃品种的RAPD分析

利用RAPD技术,用104条随机引物对甜樱桃的14个品种和中国樱桃的1个品种进行遗传多样性分析,其中有14条引物的多态性较好。用任意一条能出现扩增带的引物,能明显区分开中国樱桃和甜樱桃,RAPD标记能够准确地进行种间的区分;而用一个引物或两个引物的组合只能鉴定出甜樱桃的一个品种。聚类结果显示,中国樱桃和甜樱桃的遗传距离最远;黄色果肉的养老和其他红色果肉的品种遗传距离较远。RAPD分析基本能够反映甜樱桃品种间的遗传多样性,但效果不理想,鉴定品种较为困难。

RAPD Analysis of 15 Cherry Cultivars

RAPD analysis was used for analyzing the genetic diversity of 14 cultivars of sweet cherry and 1 cultivar of Chinese cherry. There were 14 random primers whose polymorphism of amplified bands was better. The results showed that Chinese cherry (Prunus pseudocerasus L.) could be distinguished exactly from sweet cherry (P. avium L.) by using one of the random primers. RAPD markers could be used to differentiate sweet cherry and Chinese cherry. Using one or two primers could differentiate one cultivar in the 14 tested cultivars. The clustering result showed that the genetic distance of Chinese cherry and sweet cherry was the most distant,the distance was farther between Elton (yellow fleshed) and other red fleshed cultivars. RAPD analysis could show the genetic relationship of sweet cherry cultivars,but the effectiveness was not good enough since there was difficulty in identifying sweet cherry cultivars.