| 高效液相色谱-蒸发光散射检测器测定茶叶中茶氨酸 |
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| 引用本文： | 李银花,刘仲华,黄建安,龚雨顺.高效液相色谱-蒸发光散射检测器测定茶叶中茶氨酸[J].茶叶科学,2005,25(3):225-228. |
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| 作者姓名： | 李银花 刘仲华 黄建安 龚雨顺 |
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| 作者单位： | 湖南农业大学天然产物研究中心,湖南,长沙,410128;湖南农业大学天然产物研究中心,湖南,长沙,410128;湖南农业大学天然产物研究中心,湖南,长沙,410128;湖南农业大学天然产物研究中心,湖南,长沙,410128 |
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| 摘 要： | 建立未衍生化高效液相色谱-蒸发光散射检测器测定茶叶中茶氨酸。采用Polaris-C18(250mm×4.6mm)色谱柱,以含0.1%(体积分数)三氟乙酸的水和0.1%(体积分数)三氟乙酸的乙腈作梯度洗脱,流速0.5ml/min,蒸发光散射检测器为ELSD-2000。茶氨酸浓度在0.062~0.450mg/ml内,其峰面积的常用对数与进样量的常用对数呈良好的线性关系,平均回收率为97.5%。本方法具有精确、灵敏等特点。
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| 关 键 词： | 高效液相色谱 蒸发光散射检测器 茶氨酸 茶叶 |
| 文章编号： | 1000-369X（2005）03-225-04 |
| 收稿时间： | 2004-11-29 |
| 修稿时间： | 2005-01-28 |
Determination of Theanine in Tea by Reversed-Phase High Performance Liquid Chromatography-Evaporative Light-Scattering Detector |
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| LI Yin-hua,LIU Zhong-hua,HUANG Jia-nan,GONG Yu-shun.Determination of Theanine in Tea by Reversed-Phase High Performance Liquid Chromatography-Evaporative Light-Scattering Detector[J].Journal of Tea Science,2005,25(3):225-228. |
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| Authors: | LI Yin-hua LIU Zhong-hua HUANG Jia-nan GONG Yu-shun |
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| Abstract: | High performance liquid chromatography (HPLC) coupled with evaporative light scattering detector (ELSD-2000)was used for the determination of theanine in tea without derivatization.A Polaris-C18 column (250 mm×4.6 mm) was used with the mobile phase of 0.1%trifluoroacetic acid water solution and 0.1% trifluoroacetic acid acetonitrile solution.The flow rate was at 0.5 ml/min.The linear response ranged from 0.062~0.450 mg/mL for theanine.The recovery was 97.5%.The method was evaluated as accurate and simple. |
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| Keywords: | HPLC Evaporative light-scattering detector Theanine Tea |
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