| 诱导培养基对水稻胚愈伤组织形成和分化的影响 |
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| 引用本文： | 卢勤,陈璋,朱秀英.诱导培养基对水稻胚愈伤组织形成和分化的影响[J].福建农林大学学报(自然科学版),1989(4). |
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| 作者姓名： | 卢勤 陈璋 朱秀英 |
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| 作者单位： | 福建农学院作物遗传育种研究所，福建农学院作物遗传育种研究所，福建农学院作物遗传育种研究所 |
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| 摘 要： | 将10个籼稻品种(系)的胚分别接种于附加2,4-D(2mg/l)的4种诱导培养基上,研究其对盾片愈伤组织形成和绿苗再生的影响。结果表明:(1)10个品种(系)的盾片愈伤组织的开始形成时间,形成高峰和频率差异显著,但同一品种对不同培养基的反应差异不明显。(2)在绿苗再生率方面,各品种对4种培养基的反应不同,培养基相同,不同品种间愈伤组织再生绿苗频率存在显著差异。总之,不同基因型的水稻,其盾片愈伤组织的诱导与绿苗再生需要采用不同的培养基,才能获得理想的结果。
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| 关 键 词： | 培养基 水稻 愈伤组织 基因型 |
THE EFFECT OF INDUCTION MEDIA ON CALLUS FORMATION AND PLANTLET REGERATION IN RICE TISSUE CULTURE |
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| Lu Qin,Chen Zhang and Zhu Xiuying.THE EFFECT OF INDUCTION MEDIA ON CALLUS FORMATION AND PLANTLET REGERATION IN RICE TISSUE CULTURE[J].Journal of Fujian Agricultural and Forestry University,1989(4). |
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| Authors: | Lu Qin Chen Zhang and Zhu Xiuying |
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| Abstract: | The effect of callus induction and plantlet regeneration in rice embryo culture were studied by using four induction media. The results are as follows:(1) The time and summit of scutellum callus formation has evident difference. The freqency of callus formation is significantly at the 5 % probability level comparing among various cultivars. (2) The frequency of plantlet regeneration has difference, too. Some cultivars are significantly at the 5 % probability level, but some cultivars are nonsignificant comparing among various media. The percentage of plantlet regeneration is significant at the 5 % probability level comparing among various cultivars. Callus induction and plantlet regeneration of rice embryo from different genotypes require suitable medium differently. |
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| Keywords: | medium rice callus genotype |
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