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早熟桃胚愈伤组织的诱导与保持
引用本文:刘杰俊,郑君强,罗筱玉,陆远平,陈桂信,赖钟雄.早熟桃胚愈伤组织的诱导与保持[J].亚热带农业研究,2005,1(4):21-24.
作者姓名:刘杰俊  郑君强  罗筱玉  陆远平  陈桂信  赖钟雄
作者单位:南平农业学校,福建,建阳,354200;三明农业学校,福建,沙县,365500;福建农林大学园艺学院,福建,福州,350002
摘    要:以早熟桃品种西选一号胚为外植体,对愈伤组织的诱导、继代保持和分化进行了研究。结果表明:2,4-D浓度为2.0mg.L-1的MS培养基是愈伤组织诱导的最适宜培养基,诱导率最高,达90.2%,诱导的愈伤组织质量好;将2,4-D浓度降低至1.0 mg.L-1,采用MS、WPM 2种培养基进行交替继代,可以使愈伤组织长期保持,但这种愈伤组织不能分化出芽。

关 键 词:早熟桃  成熟胚  愈伤组织
文章编号:1673-0925(2005)04-0021-04
收稿时间:2005-09-05
修稿时间:2005年9月5日

Induction and maintenance of the calli for the embryos of the early peach
LIU Jie-jun,ZHENG Jun-qiang,LUO Xiao-yu,LU Yuan-ping,CHEN Gui-xin,LAI Zhong-xiong.Induction and maintenance of the calli for the embryos of the early peach[J].Subtropical Agriculture Research,2005,1(4):21-24.
Authors:LIU Jie-jun  ZHENG Jun-qiang  LUO Xiao-yu  LU Yuan-ping  CHEN Gui-xin  LAI Zhong-xiong
Abstract:The calli from the embryos of early peach Xixuan 1 were induced and maintained.The results showed that the MS medium added 2,4-D was optimcal for induction of calli,whose inducing rate of calli was the highest(90.2%),the quality of calli was better than the other medium;that the calli sub-cultured alternately on MS/WPM medium whose concentration of 2,4-D was decreased to 1.0 mg·L~(-1) in inducing media could be maintained for long time,but the buds didn't differentiate from the calli sub-cultured.
Keywords:early peach  mature embryo  callus
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