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| 高坡红米的SSR遗传多样性分析 | |
| 引用本文： | 马琳,张上都,余显权,赵福胜,杨志文.高坡红米的SSR遗传多样性分析[J].种子,2009,28(11). |
| 作者姓名： | 马琳 张上都 余显权 赵福胜 杨志文 |
| 作者单位： | 1. 贵州大学生命科学院,贵阳,550025 2. 贵州大学农学院,贵阳,550025 3. 贵州大学教学实验场,贵阳,550025 4. 贵州大学农学院,贵阳,550025;贵州大学水稻研究所,贵阳,550025 5. 花溪高坡乡农业服务中心,贵阳,550034 |
| 基金项目： | 贵阳市科技局;花溪三个特色产业关键技术研究;筑科农;合同字第4-011号，贵州省科技厅;水稻种质资源创新和超级品种选育;合同号:黔科合NZ字[2005]3005-01 |
| 摘 要： | 利用145对SSR引物,对22份高坡红米材料及典型的籼粳稻品种93-11和日本晴进行遗传多样性分析,发现有13个标记具有多态性,共检测出57个等位基因变异,每对引物可检测2～10个等位变异,平均为4.3846个,平均Nei基因多样性指数(He)为0.521,范围为0.285 4(RM 18)～0.7674(RM 5414).用UPCMA聚类进行分析,在相似系数为0.52处可将高坡红米品种分为两大类,即籼稻和粳稻;PCO三维空间图揭示,所有供试材料在图中的分布与UPGMA聚类结果完全吻合,两种分析方法相互得到了印证. |
| 关 键 词： | 红米 遗传多样性 |
Analysis on SSR Genetic Diversity of Gaopo Red Rice |
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| MA Lin,ZHANG Shang-du,YU Xian-quan,ZHAO Fu-sheng,YANG Zhi-wen.Analysis on SSR Genetic Diversity of Gaopo Red Rice[J].Seed,2009,28(11). | |
| Authors: | MA Lin ZHANG Shang-du YU Xian-quan ZHAO Fu-sheng YANG Zhi-wen |
| Abstract: | A total of 145 SSR ,arlers were used to compare the genetic diversity in 22 red rices.93-11 and Nipponbare.Of the 145 SSR loci,13 polymorphic were revealed,and detected 57 alleles.The mean number of alleles per locus(N_A)was 4.3846 with a range from 2 to 10.Total genetic diversity index of Nei(He)varied greatly among loci from 0.285 4 at RM 18 to 0.767 4 at RM 5414,with an average value of 0.521.UPGMA cluster analysis showed that 24 materials were divided into 2 groups at genetic similarity coetfieient 0.52.The results of PCO and UPGMA cluster revealed that the genetic structure was multiplex. |
| Keywords: | SSR red rice SSR genetic diversity |
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