| 快速离体培养万寿菊植株叶片组织 |
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| 引用本文： | 林淦,周建平.快速离体培养万寿菊植株叶片组织[J].江西农业学报,2006,18(3):92-93. |
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| 作者姓名： | 林淦 周建平 |
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| 作者单位： | 襄樊学院,化学与生物科学系,湖北,襄樊,441053 |
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| 基金项目： | 襄樊学院基金项目(KJ04043) |
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| 摘 要： | 取万寿菊植株生长状态的叶片组织作为外植体,无菌条件下接种于MS+1.2mg/L 6-BA+0.9mg/LNAA的培养基中培养7d,叶片细胞逐渐增殖培养生成芽苗;将芽苗切割分离接种于MS+0.6mg/L6-BA+1.0mg/LNAA培养基中进行无菌培养,5d后,芽苗周围诱导出万寿菊幼苗;将万寿菊幼苗转接入含植物激素0.5mg/L NAA的MS培养基中,培养6d后,幼苗诱导生根,形成完整的万寿菊组织培养植株。
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| 关 键 词： | 万寿菊植株 叶片组织 离体培养 |
| 文章编号： | 1001-8581（2006）03-0092-02 |
| 收稿时间： | 2006-12-18 |
| 修稿时间： | 2005年12月18 |
Study on Rapid Tissue-culture of Isolated Laminae from Tagetes erecta |
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| LIN Gan,ZHOU Jian-ping.Study on Rapid Tissue-culture of Isolated Laminae from Tagetes erecta[J].Acta Agriculturae Jiangxi,2006,18(3):92-93. |
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| Authors: | LIN Gan ZHOU Jian-ping |
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| Abstract: | The explants of laminae from Tagetes erecta were cultivated.The results showed that the buds could be directly induced from laminae after cultivating them on MS 1.2mg/L 6-BA 0.9mg/L NAA medium for 7 days.The buds were inoculated on MS 0.6mg/L 6-BA 1.0mg/L NAA medium and could form many clumped sprouts and then differentiate gradually into plantlets after 5 days.The excised plantlets could produce root after being cultivated on MS medium with 0.5mg/L NAA for 6 days,and then develop into normal plants successfully. |
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| Keywords: | Tagetes erecta Lamina tissue Tissue-culture in vitro |
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