| 外源DNA完全酶解成分在水稻浸胚法导入中的诱变作用 |
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| 引用本文： | 张学文,唐湘如,董延瑜. 外源DNA完全酶解成分在水稻浸胚法导入中的诱变作用[J]. 湖南农业大学学报(自然科学版), 1994, 0(6) |
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| 作者姓名： | 张学文 唐湘如 董延瑜 |
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| 作者单位： | 湖南农业大学生物技术系，湖南农业大学农学系 |
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| 摘 要： | 外源ＤＮＡ以ＤＮＡ酶（ＤＮａｓｅⅠ）完全酶解后，浸泡处理灿型中优早２号糙米，研究了外源ＤＮＡ酶解成分在水稻浸胚法导入中的诱变作用。处理当代即表现了一定程度的变异，表明浸胚法外源ＤＮＡ导入过程中确存在着完全降解成分的诱变作用。
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| 关 键 词： | 外源，脱氧核糖核酸，水稻，浸泡法，酶水解／诱变 |
THE MUTATION-INDUCING FUNCTION OF COMPLETELY DIGESTED DNA DURING THE EMBRYO-SOAKING INTRODUCING OF EXOGENOUS DNA INTO RICE |
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| Zhang Xuewen Tang Xiangru DongYanyu. THE MUTATION-INDUCING FUNCTION OF COMPLETELY DIGESTED DNA DURING THE EMBRYO-SOAKING INTRODUCING OF EXOGENOUS DNA INTO RICE[J]. Journal of Hunan Agricultural University, 1994, 0(6) |
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| Authors: | Zhang Xuewen Tang Xiangru DongYanyu |
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| Abstract: | The mutation-inducing function of the elements of the completely digested DNA treated with nuclease(DNase 1) was studied by means of soaking the rice grains of Zhongyouzao 2 with the digested DNA solution.Mutations found at some extant in the soaked generation.It showed that some mutations were induced by the elements of complete-ly digested DNA during embryo-soaking introducing of foreign DNA into rice. |
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| Keywords: | exogenous deoxyribonucleic paddy infusion methods enzyme hydrolysis /mutation-inducing |
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