我国主要地方绵羊品种遗传亲缘关系

利用 10种 10碱基的随机引物 ,分析了我国 8个地方绵羊品种计 88只绵羊的随机扩增多态 DNA。结果表明 ,我国地方绵羊品种基因组 DNA多态位点百分率为 81.36 % ,群体平均遗传多样性指数为 1.3370 ,具有丰富的群体遗传多样性。但滩羊、小尾寒羊、藏绵羊和蒙古羊群体遗传多样性程度较低 ,应加强保种措施。群体遗传分化指数为0 .9172 ,说明遗传变异主要存在于群体间。品种间的分子聚类关系基本上反映了品种间的遗传亲缘关系 ,与品种的形成历史及我国地方绵羊的起源进化学说基本一致 ,具有相同来源的蒙古羊、乌珠穆沁羊、小尾寒羊和湖羊的分子聚类关系表明 ,4个地方绵羊品种间已经有了明显的遗传分化 ,小尾寒羊、乌珠穆沁羊间遗传分化较低 ,湖羊与蒙古羊之间相对较高 ,而小尾寒羊和乌珠穆沁羊与湖羊和蒙古羊之间的遗传分化最高

Random Amplified Polymorphic DNA of Chinese Main Indigenous Sheep Breeds

The random amplified polymorphic DNA(RAPD) of 88 individuals from 8 Chinese indigenous sheep breeds was studied using 10 arbitrary primers with 10 base pairs.The results showed that the percentage of polymorphic loci and the average index of genetic diversity for whole population is 81.36% and (1.337 0) respectively.It could be inferred that Chinese indigenous sheep breeds are with abundant genetic diversity.But preservation should be carried out for Tan sheep,Small Tail Han sheep,Tibetan sheep and Mongolia sheep due to their deficiency of genetic diversity.The index of genetic differentiation of whole population is (0.917 2).It could be concluded that the genetic variation exists mainly between populations.The molecular dendrogram among sheep populations illuminate the genetic relationship of populations seems to be in accord with their forming history,origin and evolution.The dendrogram among some sheep breeds indicate their same origination,and that the genetic differentiation among 4 indigenous sheep breeds is evident,and the genetic differentiation between Hu sheep and Mongolia sheep is higher than that between Small Tail Han sheep and Ujumqin sheep,but is the highest between the two groups.