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叶尔羌高原鳅外周血淋巴细胞的分离
引用本文:沙爱龙,贾山岭,潘伊薇,刘书东,李莲瑞. 叶尔羌高原鳅外周血淋巴细胞的分离[J]. 畜牧与饲料科学, 2012, 33(9): 6-6,8
作者姓名:沙爱龙  贾山岭  潘伊薇  刘书东  李莲瑞
作者单位:塔里木大学动物科学学院新疆生产建设兵团塔里木畜牧科技重点实验室,新疆阿拉尔,843300
基金项目:新疆生产建设兵团博士资金项目
摘    要:
以体质健康的叶尔羌高原鳅为研究对象,分离其外周血淋巴细胞。结果表明,采用浓度为1.085g/mL的淋巴细胞分离液在2000r/min离心35min的情况下,可将叶尔羌高原鳅外周血淋巴细胞有效地分离出来。该研究不仅为叶尔羌高原鳅淋巴细胞功能研究提供了依据,还可为叶尔羌高原鳅的疾病防治、人工养殖提供重要参考。

关 键 词:叶尔羌高原鳅  外周血  淋巴细胞  分离

The Separation of Peripheral Blood Lymphocyte of Triplophysa(Hedinichthys) yarkandensis Day
SHA Ai-long,JIA Shan-ling,PAN Yi-wei,LIU Shu-dong,LI Lian-rui. The Separation of Peripheral Blood Lymphocyte of Triplophysa(Hedinichthys) yarkandensis Day[J]. Animal Husbandry and Feed Science, 2012, 33(9): 6-6,8
Authors:SHA Ai-long  JIA Shan-ling  PAN Yi-wei  LIU Shu-dong  LI Lian-rui
Affiliation:(College of Animal Science,Key Laboratory of Tarim Animal Husbandry Science and Technology of Xinjiang Production and Construction Corps,Tarim University,Alar 843300,China)
Abstract:
Healthy Triplophysa(Hedinichthys) yarkandensis Day was designed as the research object which isolated from peripheral blood lymphocyte.The results showed that lymphocytes of peripheral blood of Triplophysa(Hedinichthys) yarkandensis could be separated by the density 1.085 g/mL separation medium at 2 000 r/min centrifugation for 35 min.It could provide reference information for the disease control and prevention,artificial breeding of Triplophysa(Hedinichthys) yarkandensis.
Keywords:Triplophysa(Hedinichthys) yarkandensis Day  peripheral blood  lymphocyte  separation
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