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| 玫瑰黄链霉菌Men-myco-93-63 nsdA_(mgh)基因的验证 | |
| 引用本文： | 沈凤英,吴伟刚,冀红柳,李亚宁,刘大群.玫瑰黄链霉菌Men-myco-93-63 nsdA_(mgh)基因的验证[J].河南农业科学,2010(3). |
| 作者姓名： | 沈凤英 吴伟刚 冀红柳 李亚宁 刘大群 |
| 作者单位： | 1. 河北农业大学,植物保护学院,河北省植物病虫害生物防治工程技术研究中心,河北,保定,071001;河北北方学院,河北,张家口,075000 2. 河北北方学院,河北,张家口,075000 3. 河北农业大学,植物保护学院,河北省植物病虫害生物防治工程技术研究中心,河北,保定,071001 |
| 基金项目： | 国家自然科学基金项目，国家高技术研究与发展计划(863)，河北省自然科学基金项目 |
| 摘 要： | 玫瑰黄链霉菌(Streptomyces roseoflavus)Men-myco-93-63是分离自马铃薯疮痂病自然衰退土壤中的一株拮抗菌。该菌株及其发酵液对棉花黄萎病菌(Verticillium dahliae)等多种重要的植物病原真菌都具有较强的抑制作用。nsdA(negative regulator of Streptomyces differentiation)基因是从天蓝色链霉菌中发现的与抗生素合成相关的负调控基因。根据天蓝色链霉菌A3(2)的序列设计引物,PCR扩增出Men-myco-93-63nsdAmgh基因,经斑点杂交法验证,nsdAmgh确实在该生防菌中存在。该基因的发现有助于进一步了解该生防菌产生抗生素调节的分子机制,也为抗生素高产菌株的获得提供了新的靶标。 |
| 关 键 词： | 玫瑰黄链霉菌Men-myco-93-63 nsdA基因 斑点杂交 核酸探针 |
The Validation of nsdAmgh Gene in Streptomyces roseoflavus Men-myco-93-63 |
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| SHEN Feng-ying,WU Wei-gang,JI Hong-liu,LI Ya-ning,LIU Da-qun.The Validation of nsdAmgh Gene in Streptomyces roseoflavus Men-myco-93-63[J].Journal of Henan Agricultural Sciences,2010(3). | |
| Authors: | SHEN Feng-ying WU Wei-gang JI Hong-liu LI Ya-ning LIU Da-qun |
| Abstract: | S.roseoflavus Men-myco-93-63 is an antagonistic strain isolated from potato scab disease induced naturally decaying soil.The strain and its fermentation fluid can effectively inhibit many pathogenic plant fungi,such as V.dahliae,thereby control the corresponding plant diseases.nsdA (negative regulator of Streptomyces differentiation ) is a globally negative regulator gene first found in S.coelicolor.nsdA mgh gene of Men-myco-93-63 amplified by PCR using the primer sequences designed for S.coelicolor A3(2) was verified by dot-blot hybridization in this study.The detection of nsdA mgh gene will contribute to the understanding of antibiotic regulation mechanism and provide a new way to obtain high-yield antibiotic producing strain of S.roseoflavus Men-myco-93-63. |
| Keywords: | Streptomyces roseoflavus Men-myco-93-63 nsdA gene Dot-blot hybridization Nucleicacid probe |
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