| 转双基因(Bt/Pi9)水稻的分子检测及抗性鉴定 |
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| 引用本文： | 杨塞. 转双基因(Bt/Pi9)水稻的分子检测及抗性鉴定[J]. 长江大学学报, 2008, 5(4) |
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| 作者姓名： | 杨塞 |
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| 作者单位： | 湖南农业大学东方科技学院,湖南,长沙,410128 |
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| 摘 要： | 以已转入抗虫Bt基因和分子标记的抗稻瘟病基因Pi9的超级稻亲本双价M86为实验材料，通过分子检测和田间农艺性状鉴定，结果显示Bt在5个株系中基因能够稳定遗传，而Pi9基因在3个株系中丢失，在2个株系中能够稳定遗传。含有B￡基因和Pi9基因的植株体，能够对螟虫和稻瘟病产生抗性。
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| 关 键 词： | 水稻 分子检测 抗性鉴定 |
Molecular Detection of Double-transgenic(Bt/Pi9) Hybrids and Evaluation of Their Resistance to Diseases |
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| YANG Sai. Molecular Detection of Double-transgenic(Bt/Pi9) Hybrids and Evaluation of Their Resistance to Diseases[J]. Journal of Yangtze University, 2008, 5(4) |
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| Authors: | YANG Sai |
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| Affiliation: | YANG Sai (College of Oriental Science , Technology,Hunan Agricultural University,Changsha,Hunan 410128,China) |
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| Abstract: | Taking super rice restoring lines M86(contain both Bt and Pi9)as experimental materials,through molecular detection and resistance identification,the result showed that Bt gene could be transmitted into the descendants in 5 lines,but Pi9 gene was lost in 3 lines. The genome of these plants with Bt gene and Pi9 gene could be produced with resistance to the stem borer and blast. |
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| Keywords: | hybrids molecular assessment resistance identification |
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