| 光活化杀虫植物的1种新型筛选方法 |
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| 引用本文： | 田永清,庄流东,徐汉虹.光活化杀虫植物的1种新型筛选方法[J].华中农业大学学报,2008,27(3). |
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| 作者姓名： | 田永清 庄流东 徐汉虹 |
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| 作者单位： | 天然农药与化学生物学教育部重点实验室/华南农业大学资源环境学院,广州,510642 |
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| 基金项目： | 国家自然科学基金
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天然农药与化学生物学教育部重点实验室开放基金 |
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| 摘 要： | 用二苯氨基脲与50种植物的提取物分别进行了反应,含有光敏毒素的提取物与二苯氨基脲在光照条件下反应时能发生颜色改变.根据二苯氨基脲能被活性氧氧化成红色产物的原理,建立了光活化杀虫植物的新型筛选方法,此方法比经典的生物活性测定方法简单、快速、灵敏.
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| 关 键 词： | 筛选方法 二苯氨基脲 光活化杀虫 植物 |
A New Screening Method for Photo-Activated Insecticidal Plants |
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| TIAN Yong-qing ZHUANG Liu-dong XU Han-hong.A New Screening Method for Photo-Activated Insecticidal Plants[J].Journal of Huazhong Agricultural University,2008,27(3). |
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| Authors: | TIAN Yong-qing ZHUANG Liu-dong XU Han-hong |
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| Institution: | Key Laboratory of Natural Pesticides and Chemical Biology;Ministry of Education/College of Resources and Environment;South China Agricultural University;Guangzhou 510642;China |
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| Abstract: | Diphenylcarbazide was applied to react with 50 plant extracts respectively,and color change could be seen if the extract contained photoxins when the reaction was in the presence of ultraviolet.According to the fact that diphenylcarbazide could be oxidized to red product by reactive oxygen species,a new screening method for photo-activated insecticidal plants was established,which was perationally simpler,more rapid and sensitive than the conventional bioassay. |
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| Keywords: | screening method diphenylcarbazide photo-activated insecticidal plants |
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